广西科学2024，Vol.31Issue(2)：380-388,9.DOI:10.13656/j.cnki.gxkx.20240619.018
消瘤藤总香豆素诱导结直肠癌细胞铁死亡的作用研究
Study on the Effect of Total Coumarins from Pileostegia tomen-tella Inducing Ferroptosis of Colorectal Cancer Cells
摘要
Abstract
Total Coumarins from Pileostegia tomentella(TCPT)was a traditional Yao medicine in Guangxi,to explore the effect and mechanism of TCPT in inducing ferroptosis of colorectal cancer HT-29 cells,human Colorectal Cancer(CRC)HT-29 cells were treated with different concentrations of TCPT in this study,and the changes of cell viability were detected by Cell Counting Kit-8(CCK-8)combined with Flow Cytometry(FCM).The morphological changes of mitochondria were observed by transmission electron microscopy,and the changes of intracellular iron-dependent Reactive Oxygen Species(ROS)content were detected by di-chlorofluorescein(DCFH-DA)dye probe method.The specific fluorescent probe BODIPY 581/589 C11 was used to detect intracellular lipid peroxide content.The expression levels of ferroptosis-related proteins Gluta-thione Peroxidase 4(GPX4),Nuclear receptor Coactivator 4(NCOA4),Cysteine-glutamate antiporter sub-unit(xCT)and Divalent Metal Transporter 1(DMT1)proteins in HT-29 cells were detected by Western blotting.The results showed that after TCPT treatment,the cell viability of human colorectal cancer HT-29 cells decreased in a concentration-dependent manner.Cell mitochondria showed typical ferroptosis morpho-logical characteristics such as cytoplasmic sudden reduction,ridge collapse,and'concentric circles'.The levels of intracellular ROS and lipid peroxides in the cells were significantly increased in a dose-dependent manner.The expression levels of ferroptosis marker proteins GPX4 and xCT in HT-29 cells were down-reg-ulated,while the expression levels of NCOA4 and DMT1 were up-regulated.In summary,the total couma-rin from Pileostegia tomentella can significantly increase the levels of intracellular ROS and lipid peroxida-tion,thus inducing ferroptosis in colorectal cancer HT-29 cells,which may be one of its anti-colorectal canc-er mechanisms.关键词
瑶药/消瘤藤/总香豆素/铁死亡/结直肠癌Key words
Yao medicine/Pileostegia tomentella/total coumarins/ferroptosis/Colorectal Cancer(CRC)分类
医药卫生引用本文复制引用
黎骊,邓聿胤,吕纪华,陆国寿,刘瑛..消瘤藤总香豆素诱导结直肠癌细胞铁死亡的作用研究[J].广西科学,2024,31(2):380-388,9.基金项目
广西自然科学基金青年科学基金项目(2022GXNSFBA035500),广西中医药适宜技术开发与推广项目(GZSY2024010)和广西中医药重点学科建设项目(GZXK-Z-20-75)资助. （2022GXNSFBA035500）
