福建农业科技2024，Vol.55Issue(4)：17-24,8.DOI:10.13651/j.cnki.fjnykj.2024.04.003
双孢蘑菇栽培基质二次发酵过程真菌群落结构变化分析
Change Analysis of Fungal Community Structure During the Secondary Fermentation Process of Agaricus bisporus Cultivation Substrate
摘要
Abstract
In order to clarify the microbial dynamics and diversity during the secondary fermentation of Agaricus bisporus cultivation substrate,the high-throughput sequencing technology was used to determine the changes of fungal community structure during the secondary fermentation of Agaricus bisporus cultivation substrate,and to explore the relationship between community composition and environmental factors.The results showed that the total number of fungal taxonomic units(OTUs)in the four fermentation stages of the secondary fermentation process of Agaricus bisporus cultivation substrate was 999,including 10 phyla,26 classes,56 orders,126 families,240 genera,and 403 species.Among them,the species of Streptococcus thermophilus(12.72%-42.63%),unclassified_f_Sortariaceae(0.12%-31.63%),Candida(1.30%-17.32%),Aspergillus(2.57%-8.98%),Penicillium(2.88%-9.76%),and Kernia(0.89%-4.18%)occupied the dominant position.During the fermentation process,the abundance of fungi belonging to genera such as Streptococcus thermophilus,Candida,Aspergillus,and Penicillium increased at the early and middle stages of fermentation,and then decreased at the later stage.The relative abundance of Streptococcus thermophilus and Penicillium reached the peak in the middle stage of fermentation.Ammonia emission and pile temperature were the key indicators of fermentation end point.The correlation between fungal community and ammonia emission and pile temperature was analyzed by using the redundancy analysis.The results showed that the abundance of Streptococcus thermophilus,Penicillium and Candida was positively correlated with the fermentation temperature and ammonia emission.The higher the abundance was,the shorter the secondary fermentation cycle was and the better the fermentation quality was.关键词
双孢蘑菇/栽培基质/二次发酵/真菌多样性Key words
Agaricus bisporus/Cultivation substrate/Secondary fermentation/Fungal diversity分类
园艺学与植物营养学引用本文复制引用
陈德局,陈梅春,蓝江林,王阶平,张海峰..双孢蘑菇栽培基质二次发酵过程真菌群落结构变化分析[J].福建农业科技,2024,55(4):17-24,8.基金项目
福建省科技计划省属公益类项目(2020R1034005). （2020R1034005）
