海南医学院学报2024，Vol.30Issue(12)：921-929,9.DOI:10.13210/j.cnki.jhmu.20240329.002
归脾汤对化疗肌无力大鼠抗炎机制的实验研究
Experimental study on the anti-inflammatory mechanism of Guipi decoction on myasthenia rats after chemotherapy
摘要
Abstract
Objective:To investigate the anti-inflammatory mechanism of Guipi decoction in improving chemotherapy-induced myasthenia based on the results of network pharmacology and bioinformatics analysis.Methods:Forty SPF male SD rats were ran-domly divided into the blank group,the model group,the Guipi decoction low-dose,medium-dose and high-dose groups with 8 rats in each group according to random number table method.5-Fluorouracil(28 mg/kg)was intraperitoneally injected for 5 consecu-tive days to establish a chemotherapy-induced muscle weakness model.Guipi decoction at low,medium and high dose were admin-istrated by gavage(0.5,1,2 g/mL)for 14 consecutive days.The body weight and grip strength of each group were recorded,and the expression of reactive oxygen species(ROS)in gastrocnemius tissues was detected by immunofluorescence.The expressions of hypoxia-induced-factor 1α(HIF-1α)protein in gastrocnemius was detected by immunohistochemistry.The expression of inflam-matory factors IL-1β,IL-6,TNF-α,chemokine receptors CCR1,CCR2,CCR7,CXCR-4 and TNF-family receptors TNFR1 and TRAF6 in gastrocnemius were detected by Western blot.Results:Compared with the control group,the body weight and grip strength of rats in the model group were significantly decreased(P<0.01).ROS production in gastrocnemius was significant-ly increased(P<0.01).Immunohistochemistry results showed that HIF-1α expression was increased(P<0.01).The expressions of IL-1β,IL-6,TNF-α,CCR1,CCR2,CCR7,CXCR-4,TNFR1 and TRAF6 were significantly increased(P<0.01).Com-pared with the model group,ROS production in gastrocnemius of low,medium and high dose Guipi decoction groups was signifi-cantly decreased(P<0.01),while the expressions of HIF-1α were significantly increased(P<0.01).The expressions of IL-1β,IL-6,TNF-α,CCR1,CCR2,CCR7,CXCR-4,TNFR1 and TRAF6 were significantly decreased(P<0.05).Conclusion:Gui-pi decoction may inhibit the level of intramusculature inflammation and induce the activation of HIF-1α to combat the oxidative stress caused by inflammation,so as to alleviate the muscle weakness caused by chemotherapy.关键词
化疗/肌无力/归脾汤/炎症/TNF家族受体/趋化因子受体Key words
Chemotherapy/Myasthenia/Guipi decoction/Inflammation/TNF family receptors/Chemokine receptors分类
医药卫生引用本文复制引用
宋予馨,雷萍,魏东升,王艺,马贤德..归脾汤对化疗肌无力大鼠抗炎机制的实验研究[J].海南医学院学报,2024,30(12):921-929,9.基金项目
This study was supported by National Natural Science Foundation of China(82374375) （82374375）
Liaoning Provincial Science and Technology Department Nature Fund Guidance Program Project(2019-ZD-0444)国家自然科学基金面上项目(82374375) （2019-ZD-0444）
辽宁省科技厅自然基金指导计划项目(2019-ZD-0444) （2019-ZD-0444）
