河南农业大学学报2024，Vol.58Issue(3)：383-393,11.DOI:10.16445/j.cnki.1000-2340.20240425.003
固始鸡胸肌代谢组特征评价及相关基因鉴定
Evaluation of metabolome characteristics and identification of related genes in Gushi chicken breast muscle
摘要
Abstract
[Objective]This study was conducted to explore the the characterization and potential regu-latory mechanisms of excellent meat quality traits in Gushi chickens.[Method]Liquid chromatography-mass spectrometry(LC-MS)was used to characterize the breast muscle metabolites of 120-day-old Gushi chickens using 45-day-old A A broilers as a control group,and the genes associated with the differential metabolite molecules were identified in conjunction with the breast muscle transcriptome da-ta.[Result]Metabolomic analysis showed that there were 134 differentially accumulated metabolite(DAMs)molecules between the two breeds of chicken breast muscle,and 48 DAMs were significantly up-regulated and 86 DAMs were significantly down-regulated in the breast muscle of Gushi chickens compared with AA broilers,which were mainly attributed to amino acids,fatty acids and peptides.Meanwhile,based on the breast muscle transcriptome data,1 501 differentially expressed genes(DEGs)were screened,and 719 were up-regulated and 712 were down-regulated in the breast muscle of Gushi chickens compared with AA broilers.Combined metabolomic and transcriptomic analyses re-vealed that DEGs and DAMs were significantly enriched in the pathways of arginine and proline metab-olism,glycerophospholipid metabolism,glycine and serine and threonine metabolism.Correlation anal-ysis showed that 15 gene-metabolite molecules pairs were significantly positively correlated and 13 gene-metabolite molecules pairs were significantly negatively correlated,involving L-serine,4-acetami-do-2-aminobutyric acid,N2-succinyl-L-ornithine,O-phosphoethanolamine,choline and other metabo-lite molecules and genes such as GATM,SARDH,PTDSS2,ALDH7A1,AGPAT2 and EHHADH,a-mong others.[Conclusion]Compared with AA broilers,significantly upregulated differential metabo-lites such as phosphatidylethanolamine,L-serine,glutathione,myostatin,myo-inositol,and malic acid and their related differential genes are potential markers of breast muscle flavor in Gushi chickens.关键词
固始鸡/AA肉鸡/胸肌/代谢组/转录组Key words
Gushi chicken/A A broiler/breast muscles/metabolome/transcriptome分类
农业科技引用本文复制引用
李帅浩,张鸿源,代雅琪,李鸿泰,谷金星,吴冉,李国喜..固始鸡胸肌代谢组特征评价及相关基因鉴定[J].河南农业大学学报,2024,58(3):383-393,11.基金项目
神农种业实验室一流课题项目(SN01-2022-05) （SN01-2022-05）
国家自然科学基金项目(32072692) （32072692）
河南省高等教育教学改革研究与实践项目(2024SJGLX0008) （2024SJGLX0008）
