甜瓜CmGLK转录因子调控果实品质的遗传网络解析OA北大核心CSTPCD

[Objective]In order to elucidate the regulatory network of melon CmGLK transcription fac-tor regulating fruit quality and provide a theoretical basis for the improvement of melon fruit quality.[Method]The melon inbred line HB42(used as wild type)and its Cmglk near isogenic line glk-NIL(NIL)were used to conduct transcriptomic and metabolomic analysis on the pulp of mature fruits at 30 days after pollination.[Result]These metabolites related to melon fruit quality,such as oxaloacetic acid,dihydroxyacetone phosphate,3-phosphate-D-glyceroic acid,arbutin,D-glucose-1,6-diphos-phate,9,10-epoxidodecenoic acid,12,13-epoxy-9-octadecenoic acid,were all down-regulated in the NIL.Transcriptomic analysis showed that the malate dehydrogenase gene(MDH)and the E2 subunit oxy glutarate dehydrogenase 2 gene(E2-OGDHzhe2)involved in the tricarboxylic acid cycle pathway were down-regulated in the NIL.Most important genes involved in the glycolytic pathway are down-reg-ulated in the NIL,with only four genes up-regulated in the NIL,including the pyruvate dehydrogenase gene(PDHA)and chloroplast aldosterone reductase gene(CHLAKR).The key genes related to solu-ble sugars including β-glucosidase 15 gene(BGLU15),β-glucosidase 12 gene(BGLU12)are down-regulated in the NIL.Five of the nine lipoxygenase genes(LOX)involved in the linoleic acid metabo-lism pathway are up-regulated in the NIL and four of them are down-regulated in the NIL.[Conclu-sion]The CmGLK transcription factors may affect melon quality by regulating the metabolism of soluble sugars and organic acids,and the genetic regulatory network of melon CmGLK transcription factor regu-lating fruit quality was preliminarily elucidated.