河南农业大学学报2024，Vol.58Issue(3)：424-434,11.DOI:10.16445/j.cnki.1000-2340.20240305.001
甜瓜CmGLK转录因子调控果实品质的遗传网络解析
Genetic network analysis of melon CmGLK transcription factor in regulating fruit quality
摘要
Abstract
[Objective]In order to elucidate the regulatory network of melon CmGLK transcription fac-tor regulating fruit quality and provide a theoretical basis for the improvement of melon fruit quality.[Method]The melon inbred line HB42(used as wild type)and its Cmglk near isogenic line glk-NIL(NIL)were used to conduct transcriptomic and metabolomic analysis on the pulp of mature fruits at 30 days after pollination.[Result]These metabolites related to melon fruit quality,such as oxaloacetic acid,dihydroxyacetone phosphate,3-phosphate-D-glyceroic acid,arbutin,D-glucose-1,6-diphos-phate,9,10-epoxidodecenoic acid,12,13-epoxy-9-octadecenoic acid,were all down-regulated in the NIL.Transcriptomic analysis showed that the malate dehydrogenase gene(MDH)and the E2 subunit oxy glutarate dehydrogenase 2 gene(E2-OGDHzhe2)involved in the tricarboxylic acid cycle pathway were down-regulated in the NIL.Most important genes involved in the glycolytic pathway are down-reg-ulated in the NIL,with only four genes up-regulated in the NIL,including the pyruvate dehydrogenase gene(PDHA)and chloroplast aldosterone reductase gene(CHLAKR).The key genes related to solu-ble sugars including β-glucosidase 15 gene(BGLU15),β-glucosidase 12 gene(BGLU12)are down-regulated in the NIL.Five of the nine lipoxygenase genes(LOX)involved in the linoleic acid metabo-lism pathway are up-regulated in the NIL and four of them are down-regulated in the NIL.[Conclu-sion]The CmGLK transcription factors may affect melon quality by regulating the metabolism of soluble sugars and organic acids,and the genetic regulatory network of melon CmGLK transcription factor regu-lating fruit quality was preliminarily elucidated.关键词
甜瓜/转录组学/代谢组学/GLK转录因子/果实品质Key words
melon/transcriptomics/metabolomics/GLK transcription factor/fruit quality分类
园艺学与植物营养学引用本文复制引用
郭姚淼,朱华玉,段世享,刘港运,李鹏飞,康旗帅,杨森,豆峻岭,胡建斌,杨路明..甜瓜CmGLK转录因子调控果实品质的遗传网络解析[J].河南农业大学学报,2024,58(3):424-434,11.基金项目
国家自然科学基金面上项目(31872133) （31872133）
河南省自然科学基金优秀青年项目(212300410048) （212300410048）
河南省高等学校青年骨干教师培养计划项目(2020GGJS045) （2020GGJS045）
