化学工程2024，Vol.52Issue(6)：1-5,32,6.DOI:10.3969/j.issn.1005-9954.2024.06.001
高效液相色谱法检测葡萄糖酸-δ-内酯
Determination of gluconic acid-δ-lactone by high performance liquid chromatography
师保炜 1吴艳阳 1武斌 1纪利俊 1陈葵1
作者信息
- 1. 华东理工大学 化工学院,上海 200237
- 折叠
摘要
Abstract
Gluconic acid-δ-lactone is often prepared by evaporation concentration from gluconic acid aqueous solution and contains a small amount of gluconic acid and gluconic acid-γ-lactone.In order to determine gluconic acid-δ-lactone in the product,a high-performance liquid chromatography method for the rapid separation and anylysis of gluconic acid-δ-lactone,gluconic acid and gluconic acid-γ-lactone was established and the conditions were optimized for the determination of gluconic acid-δ-lactone.The Titank Hilic column was chosen for the separation of gluconic acid-δ-lactone,gluconic acid and gluconic acid-γ-lactone.The mobile phase was 0.01 mol/L acetonitrile-phosphoric acid aqueous solution(volume ratio 90:10),the column temperature was 30° C,the flow rate was 1.0 mL/min,the detection wavelength was 205 nm,and the injection volume was 20 μL.The results show that gluconic acid-δ-lac tone can be well detected within 7 min.Gluconic acid-δ-lactone presentes a good linear relationship(r＞0.999)in the mass concentration range of 0.5-2.5 mg/mL,the detection limit is 0.002 mg/mL,the limit of quantification is 0.5 mg/mL,the average spiked recovery of the three levels is 98.8％-99.9％,and the relative standard deviation is 0.1％-0.4％(n=5).This method characterizes short analysis time,high sensitivity and good separation effect,and can be used to determine gluconic acid-δ-lactone,gluconic acid and gluconic acid-γ-lactone simultaneously.关键词
高效液相色谱/二极管阵列检测器/Hilic色谱柱/葡萄糖酸-δ-内酯/葡萄糖酸/葡萄糖酸-γ-内酯Key words
high performance liquid chromatography/diode array detectors/Hilic chromatography column/gluconic acid-δ-lactone/gluconic acid/gluconic acid-γ-lactone分类
化学化工引用本文复制引用
师保炜,吴艳阳,武斌,纪利俊,陈葵..高效液相色谱法检测葡萄糖酸-δ-内酯[J].化学工程,2024,52(6):1-5,32,6.
