粤西卷羽鸡BMP6基因多态性与生长性状及屠宰性状的关联分析OA北大核心CSTPCD
Association analysis of BMP6 gene polymorphisms with growth and slaughter traits in Yuexi Frizzled Feather chicken
[目的]探讨BMP6 基因多态性对粤西卷羽鸡生长性状及屠宰性状的影响.[方法]以 120 只粤西卷羽鸡为试验材料,对BMP6 基因外显子设计特异性扩增引物,采取PCR产物直接测序法分析SNP位点,利用单因素方差分析对SNP位点基因型与生长性状及屠宰性状进行关联分析.[结果]在BMP6 基因外显子区域共检测到7 个SNP位点,其中,g.64185950 T>C、g.64195411 G>A和g.64195613 C>T处于编码区,均为同义突变.Hardy-Weinberg平衡检验结果显示,g.64195833 T>A在粤西卷羽鸡群体中显著偏离Hardy-Weinberg平衡(P<0.05).基因型与生长性状关联分析结果表明:在公鸡中,g.64195613 C>T位点CC基因型胫长显著高于TT基因型(P<0.05);在母鸡中,g.64195411 G>A位点AA基因型龙骨长显著高于AG基因型(P<0.05),g.64196373 T>C位点CC基因型胫长极显著高于CT基因型(P<0.01),g.64196735 T>C位点CT基因型胫长显著高于TT基因型(P<0.05).基因型与屠宰性状关联分析结果表明:在公鸡中,g.64196735 T>C位点CC基因型活体质量显著高于CT基因型(P<0.05);在母鸡中,g.64195613 C>T位点CC基因型腹脂率显著高于CT基因型(P<0.05),g.64196373 T>C位点CC基因型半净膛率显著高于CT基因型(P<0.05).[结论]BMP6 基因多态性与粤西卷羽鸡的部分生长性状及屠宰性状相关,可作为粤西卷羽鸡标记辅助选择的候选基因.
[Objective]To investigate the effect of BMP6 gene polymorphism on growth and slaughter traits of Yuexi Frizzled Feather chicken.[Method]A total of 120 Yuexi Frizzled Feather chickens were used as experimental materials.Specific primers were designed for the exon fragment of the BMP6 gene.The SNP sites were detected by direct sequencing of PCR products.The single factor analysis of variance was used to analyze the associations of genotypes with growth and slaughter traits for each SNP site.[Result]Seven SNP sites were detected in the exon region of BMP6 gene in Yuexi Frizzled Feather chicken,among which g.64185950 T>C,g.64195411 G>A and g.64195613 C>T were in the coding sequence region,and all these three SNPs were synonymous mutations.The results of Hardy-Weinberg equilibrium test showed that g.64195833 T>A significantly deviated from Hardy-Weinberg equilibrium(P<0.05)in the Yuexi Frizzled Feather chicken.The results of association analysis between genotypes and growth traits showed that,in cocks,the shank bone length in CC genotype of g.64195613 C>T site was significantly higher than that of TT genotype(P<0.05),and in hens,the keel length in AA genotype of g.64195411 G>A site was significantly higher than that of AG genotype(P<0.05),the tibia length in CC genotype of g.64196373 T>C site was significantly higher than that of CT genotype(P<0.01),the tibia length in CT genotype of g.64196735 T>C site was significantly higher than that of TT genotype(P<0.05).The results of association analysis between genotypes and slaughter traits showed that,in cocks,the live weight in CC genotype of g.64196735 T>C site was significantly higher than that of CT genotype(P<0.05),and in hens,the abdominal fat rate in CC genotype of g.64195613 C>T site was significantly higher than that of CT genotype(P<0.05),and the half-bore weight rate in CC genotype of g.64196373 T>C site was significantly higher than that of CT genotype(P<0.05).[Conclusion]The BMP6 gene polymorphisms are associated with some growth and slaughter traits,which can be used as a candidate gene for marker-assisted selection in Yuexi Frizzled Feather chicken.
李国;谢婷婷;张丽;王府建;李磊;宾乘峰;郭东雪;王晓彤;林君媛;林树带
广东海洋大学滨海农业学院,广东湛江 524088
畜牧业
粤西卷羽鸡BMP6基因生长性状屠宰性状单核苷酸多态性关联分析
Yuexi Frizzled Feather chickenBMP6 geneGrowth traitSlaughter traitSingle nucleotide polymorphismCorelation analysis
《华南农业大学学报》 2024 (004)
477-486 / 10
广东省农业农村厅种业振兴专项(2022-XPY-00-008)
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