华南农业大学学报2024，Vol.45Issue(4)：487-494,8.DOI:10.7671/j.issn.1001-411X.202403003
利用分子检测和基因编辑快速创制籼粳杂种亲和材料
Rapidly creating indica-japonica hybrid compatibility materials by molecular detection and genome editing
摘要
Abstract
[Objective]By molecular detection and genome editing,a rapid and precise creation of artificial hybrid-compatible lines is carried out to break the indica-japonica hybrid sterility and provide a new paradigm for realizing the utilization of indica-japonica hybrid vigor.[Method]In this study,an elite indica rice variety'Huanghuazhan'(HHZ)and an elite japonica rice variety'Zhongjia 8'(ZJ8)were used as test materials.The genotypes of the major indica-japonica hybrid sterility loci in HHZ and ZJ8 were detected by molecular detection.Then,the genome editing was carried out to knock out the major hybrid sterility gene(s)conferring HHZ-ZJ8 hybrid fertility,thereby rapidly and precisely creating indica-japonica hybrid F1 with high fertility.[Result]The HHZ-ZJ8 hybrid F1 plants showed strong hybrid vigor,but exhibited spikelet semi-sterility.According to genetic mechanism of intersubspecific hybrid sterility in indica and japonica rice,molecular detection revealed that one known major locus S5 exists in the HHZ-ZJ8 hybrids.Then,genome editing was used to knockout S5-ORF5+(killer gene)in HHZ and S5-ORF4+(partner gene)in ZJ8.Compared with the wild type,several important agronomic traits of these edited materials had no obvious changes.However,the spikelet fertility of the F1 plants derived from crossing these edited materials with wild type ZJ8 or HHZ were fully restored.[Conclusion]Rational design of indica-japonica hybrid breeding can be achieved by molecular detection and genome editing,enabling rapid cultivation of indica-japonica artificial hybrid-compatible lines and maximizing the retention of genetic diversity in indica and japonica rice.This study provides a new example for breaking theindica-japonicahybrid sterility and utilizing indica-japonica hybrid vigor.关键词
分子检测/基因编辑/杂种不育/杂种亲和/水稻Key words
Molecular detection/Genome editing/Hybrid sterility/Hybrid compatibility/Rice分类
农业科技引用本文复制引用
黎珠玉,蒋永捷,冯耀明,梅林,李顺媚,郑琳,陈乐天,刘耀光,谢勇尧..利用分子检测和基因编辑快速创制籼粳杂种亲和材料[J].华南农业大学学报,2024,45(4):487-494,8.基金项目
国家自然科学基金(31991222,32270671) （31991222,32270671）
广东省基础与应用基础研究基金自然科学基金杰出青年项目(2021B10564001) （2021B10564001）
岭南现代农业实验室科研项目(NT2021002) （NT2021002）
