宁夏某牛场致泌乳奶牛急性死亡的产气荚膜梭菌分离鉴定及其药敏试验OA北大核心CSTPCD

To explore the pathogens and antibiotic resistance of Clostridium perfringens isolates in 3 a-cute death cases of dairy cows from a cattle farm in Ningxia Hui Autonomous Region,differential medium was used to isolate and purify the pathogen in this study.The biochemical characteristic was confirmed by milk medium,and the molecular biology method was adopted to identify the pathogens'species and geno-types.In addition,27 antibiotics under 12 classes were used to detect the pathogens'antibiotic resistance.The results showed that the three isolated pathogenic bacteria exhibited β-hemolytic ring with the phenom-enon of"turbulent fermentation".The results of typing and identification showed that the three isolates were all Clostridium perfringens type A(named S1,S2,and S3).The results of antibiotic resistance showed that three strains of Clostridium perfringens type A are sensitive to ampicillin,ceftriaxone,ce-fazolin,meropenem,piperacillin,ampicillin-sulbactam,moderately sensitive to cefuroxime sodium,lin-ezolid,cefotaxime,ceftazidime,tigecycline,ciprofloxacin,vancomycin,minocycline,levofloxacin,and resistant to streptomycin,norfloxacin,Gentamicin,sulfamethoxazole,erythromycin,tetracycline,clinda-mycin,lincomycin,amikacin,aztreonam.In conclusion,this study determined Clostridium perfringens type A as the pathogen that caused the acute death of lactating cattle,and this isolated strain has certain resistance to some antibiotics.