军事医学2024，Vol.48Issue(6)：429-433,5.DOI:10.7644/j.issn.1674-9960.2024.06.005
舌下腺黏液性腺泡细胞特异性Cre重组酶基因敲入小鼠的建立和鉴定
Generation of a Cre recombinase knock-in mouse line labeling the mucous acinar cells of the sublingual gland
摘要
Abstract
Objective To generate and identify the Itgbl1(integrin beta-like)promoter-driven Cre knock-in mouse line.Methods Itgbll-Cre knock-in mice were generated using clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)gene editing.The Itgbl1-Cre mice were crossed with the Cre reporter ROSALSL-tdTomato)mice to detect the expression profile of Cre activity.The tdTomato expression pattern across tissues and cell-specific markers were used to identify the cell types of Itgbl1-expressing cells and their progeny.Results and Conclusion tdTomato was specifically expressed in mucous acinar cells of the sublingual gland,pancreatic islet cells,and gastric endocrine cells.In addition,tdTomato expression was also found in some of the neurons of the retina and brain,as well as in a few cells in the serosal layer of the intestine,articular cartilage,periosteum,and bone marrow.The first Itgbl1-Cre recombinase transgenic mouse line was established,which can specifically label the mucous acinar cells of the sublingual gland.关键词
整合素β样蛋白1/Cre重组酶/遗传细胞谱系示踪/多色免疫荧光Key words
integrin beta-like 1/Cre recombinase/genetic cell lineage tracing/multicolor immunofluorescence分类
生物科学引用本文复制引用
彭艳丽,杨冠,滕艳,粟柯糙,郎依铭,谢中良,李明月,周学涛,王庆业,汪海珍,杨晓..舌下腺黏液性腺泡细胞特异性Cre重组酶基因敲入小鼠的建立和鉴定[J].军事医学,2024,48(6):429-433,5.基金项目
国家重点研发计划(2018YFA0801104) （2018YFA0801104）
国家自然科学基金面上项目(82372721) （82372721）
