军事医学2024，Vol.48Issue(6)：474-480,7.DOI:10.7644/j.issn.1674-9960.2024.06.011
CRISPR-Cas12a在食源性致病菌检测中的应用研究进展
Advances in CRISPR-Cas12a in detection of foodborne pathogenic bacteria
摘要
Abstract
Foodborne pathogens make much difference to food safety,and troops are also at risk of infection during military operations and daily lives,so there is an urgent need for highly sensitive and rapid detection technologies.In recent years,with the continuous development of clustered regularly interspaced short palindromic repeats(CRISPR)technology,the CRISPR-Cas system with trans-cutting activity has received increasing attention.This paper outlines the basic principles of CRISPR-Cas12a-based detection technology and conventional signal readout technologies.It also analyzes the current status and developmentsof CRISPR-Cas12a in detecting foodborne pathogenic bacteria.The challenges to and prospects of this technology are also described.关键词
CRISPR-Cas12a/食源性致病菌/水和食品安全/现场检测Key words
CRISPR-Cas12a/foodborne pathogenic bacteria/water and food security/on-site testing分类
医药卫生引用本文复制引用
兰紫舒,陈天姣,李君文,金敏,杨栋..CRISPR-Cas12a在食源性致病菌检测中的应用研究进展[J].军事医学,2024,48(6):474-480,7.基金项目
国家重点研发计划重点专项(2023YFC3205800) （2023YFC3205800）
