兰州大学学报（医学版）2024，Vol.50Issue(5)：6-13,8.DOI:10.13885/j.issn.1000-2812.2024.05.002
中性粒细胞胞外诱捕网通过激活NLRP3炎症小体诱导人肺微血管内皮细胞焦亡
Neutrophil extracellular traps induce pyroptosis of human pulmonary microvascular endothelial cells by activating NLRP3 inflammasome
摘要
Abstract
Objective To investigate into the specific mechanism of neutrophil extracellular traps acting on pulmonary microvascular endothelial cells and inducing endothelial cell death.Methods Human pulmonary microvascular endothelial cells were stimulated with NETs 200 ng/mL isolated in vitro.The cell death was detected by PI staining.The pyroptosis markers were detected by Western blot and immunofluorescence.Results Compared with the control group,the death of human pulmonary microvascular endothelial cells was increased after stimulation with neutrophil extracellular traps.The expression of NLRP3 inflammasome and pyroptosis related markers GSDMD,N-GSDMD were significantly increased.Pretreatment of NLRP3 inhibi-tor MCC950 effectively weakened the activation of NLRP3 and further inhibited the occurrence of pyroptosis.Conclusion Neutrophil extracellular traps promote the pyroptosis of human lung microvascular endothelial cells by activating the NLRP3 inflammasome.关键词
中性粒细胞胞外诱捕网/肺微血管内皮细胞/NLRP3炎症小体/细胞焦亡Key words
neutrophil extracellular traps/pulmonary microvascular endothelial cells/NLRP3 inflammasome/pyroptosis分类
医药卫生引用本文复制引用
赵佩佩,朱嘉睿,李飞飞,张岑,张思功..中性粒细胞胞外诱捕网通过激活NLRP3炎症小体诱导人肺微血管内皮细胞焦亡[J].兰州大学学报（医学版）,2024,50(5):6-13,8.基金项目
国家自然科学基金资助项目(82060302,82260325) （82060302,82260325）
甘肃省教育科技创新项目(2022B-051) （2022B-051）
兰州大学医学创新发展计划资助项目(lzuyxcx-2022-168) （lzuyxcx-2022-168）
兰州大学第二医院萃英科技创新计划资助项目(CY2021-MS-A04,CY2021-QN-B02) （CY2021-MS-A04,CY2021-QN-B02）
