临床肝胆病杂志2024，Vol.40Issue(6)：1209-1214,6.DOI:10.12449/JCH240622
间充质干细胞联合免疫抑制剂对肝移植大鼠模型免疫排斥的影响
Effect of mesenchymal stem cells combined with immunosuppressants on immune rejection in a rat model of liver transplantation
摘要
Abstract
Objective To investigate the effect of mesenchymal stem cells(MSCs)combined with immunosuppressants(IS)on immune rejection in a rat model of liver transplantation.Methods F344 rats were divided into Normal group(without any intervention),PS group(injected with an equal volume of normal saline),MSC group(injected with MSC),IS group(injected with IS),and MSC+IS group(injected with MSC and IS),with 8 rats in each group.For all rats except those in the Normal group,the Kamada's double-cuff method was used to establish a model of orthotopic liver transplantation,without reconstruction of the hepatic artery.HE staining and Masson staining were performed for rat liver tissue,and the degree of liver fibrosis was analyzed;immunohistochemical experiments were used to measure the infiltration of T cells and NK cells,and immunofluorescence assay was used to analyze macrophage M2 polarization.A one-way analysis of variance was used for comparison of continuous data between multiple groups,and the least significant difference t-test was used for further comparison between two groups.The Kaplan-Meier method was used to plot survival curves,and the log-rank test was used for survival analysis.Results Compared with the PS group,the MSC+IS group had a significantly prolonged survival time(P＜0.01),and the MSC group,the IS group,and the MSC+IS group had a significant improvement in the histological structure of the liver and a significant reduction in the degree of liver fibrosis(all P＜0.000 1),as well as a significant reduction in the infiltration of NK and T cells(all P＜0.000 1)and a significant increase in the degree of macrophage M2 polarization(all P＜0.000 1).The MSC+IS group had a significantly better effect than the MSC group and the IS group.Conclusion MSCs combined with IS can improve liver histopathology,reduce inflammatory cell infiltration,promote macrophage M2 polarization,and exert an immunosuppressive effect in rats after liver transplantation.关键词
肝移植/间质干细胞/移植物排斥Key words
Liver Transplantation/Mesenchymal Stem Cells/Graft Rejection引用本文复制引用
李海涛,俞赛花,陈丽红,赖子森,刘海岩,刘红枝,沈聪龙..间充质干细胞联合免疫抑制剂对肝移植大鼠模型免疫排斥的影响[J].临床肝胆病杂志,2024,40(6):1209-1214,6.基金项目
福建省自然科学基金(2020J011163) （2020J011163）
福建省卫生健康委医学创新项目(2020CXA054) （2020CXA054）
福州市临床重点专科建设项目(20230101) The Natural Science Foundation of Fujian Province(2020J011163) （20230101）
The Medical Innovation Project of Fujian Province(2020CXA054) （2020CXA054）
Key Clinical Specialty Discipline Construction Program of Fuzhou(20230101) （20230101）
