南京师范大学学报（工程技术版）2024，Vol.24Issue(2)：59-66,8.DOI:10.3969/j.issn.1672-1292.2024.02.008
小鼠外周血单个核细胞对囊胚的免疫应答反应探究
Immunological Behavior of Peripheral Blood Mononuclear Cells on Mouse Blastocyst
摘要
Abstract
The egg is fertilized a sperm forming a zygote,and then develops to form a blastocyst with series developmental events.Blastocyst hatches out of ZP and interacts with the uterus initiating implantation.It has been shown that embryo implantation is an inflammatory process.The genetic heterogeneity of the embryo determines that the embryo will trigger an immune response in the uterus.However,the immunological properties of the embryo have yet to be resolved.To investigate the possible immune interaction between embryos and immune cells,we use peripheral blood mononuclear cells(PBMC)to evaluate the immunological effects of mouse blastocysts.10 and 20 mouse blastocysts are co-cultured with PBMC for 24 hours,and lipopolysaccharide(LPS)treatment is used as a control respectively.Results show that embryos induce PBMC proliferation,in which the proportion of T cells is reduced significantly(P<0.05).When treated by LPS,NK cells and monocytes which are innate immune cells,PBMC are induced in proliferation.Treating both LPS and embryos,NK cells and monocytes are further proliferated(P<0.05).The cytokines in medium is analyzed by ELISA.Data show that the pro-inflammatory molecule IL-6 and the anti-inflammatory molecule IL-10 are significantly increased after PBMC are cultivated with embryos(P<0.05).Interestingly,embryos can down-regulate the expression of LPS-induced TNF-α(P<0.05).Results show that blastocysts have a regulatory effect on PBMC subpopulation,enhance LPS-triggered innate immune response,and regulate the secretion of cytokines IL-6,TNF-α and IL-10.Our findings imply that mouse blastocysts do not only cause an inflammatory response in the endometrium,but also have a dynamic regulatory effect on uterine immune cells.This study provides insights for further analysis of uterus-embryo immunological interaction during implantation.关键词
囊胚/单个核细胞/共培养/免疫应答/细胞因子Key words
blastocysts/mononuclear cells/co-culture/immune response/cytokine分类
生物科学引用本文复制引用
滕亚迪,刘子涵,马梅香,安礼友..小鼠外周血单个核细胞对囊胚的免疫应答反应探究[J].南京师范大学学报（工程技术版）,2024,24(2):59-66,8.基金项目
新疆维吾尔自治区自然科学基金杰出青年科学基金项目(2022D01E09). （2022D01E09）
