农业机械学报2024，Vol.55Issue(6)：353-364,12.DOI:10.6041/j.issn.1000-1298.2024.06.037
不同空化射流条件下大豆蛋白-白藜芦醇互作结合机制与抗氧化活性研究
Effect of Cavitation Jet on Interaction and Antioxidant Activity of Soybean Protein-Resveratrol
摘要
Abstract
To investigate the effect of cavitation jet pretreatment on soybean protein isolate-resveratrol(SPI-RES)complexes,SPI was subjected to cavitation jet pretreatment(0 min,2 min,4 min,6 min,8 min and 10 min)before non-covalent binding with RES to form complexes.The binding of SPI to RES was studied through loading amount and encapsulation efficacy.The interaction and binding mechanism between SPI and RES were studied by using fluorescence spectroscopy,Fourier transform infrared spectroscopy,and molecular docking techniques.In addition,the physical and chemical properties and functional characteristics of the SPI-RES complexes were investigated,including ζ-potential,surface hydrophobicity,and antioxidant activity.The results indicated that after a certain period of cavitation jet pretreatment,SPI significantly increased the encapsulation efficacy and loading amount of RES,and the particle size and ζ-potential of complexes was decreased and increased respectively.The fluorescence spectrum indicated that the quenching of SPI by RES was static quenching,and the reaction was spontaneous.Fourier transform infrared spectroscopy indicated that appropriate cavitation jet pretreatment promoted the transition of SPI from ordered to disordered structures,thereby combining more RES.The thermodynamic parameters and molecular docking results indicated that hydrophobic interactions were the main forces and involved hydrogen bonds.In addition,after appropriate cavitation jet pretreatment,the surface hydrophobicity and antioxidant activity of SPI-RES complexes were increased.The research result can provide a preliminary theoretical basis for the exploration of the application field of cavitation jet pretreatment of soybean protein isolate and the development of fat-soluble active substances in health food.关键词
大豆分离蛋白/白藜芦醇/空化射流/非共价复合物Key words
soybean protein isolate/resveratrol/cavitation jet/non-covalent complexes分类
轻工纺织引用本文复制引用
王中江,孙福伟,任双鹤,沈路路,张硕,郭增旺..不同空化射流条件下大豆蛋白-白藜芦醇互作结合机制与抗氧化活性研究[J].农业机械学报,2024,55(6):353-364,12.基金项目
国家自然科学基金项目(32202228)、山东省重点研发计划项目(2022CXGC010603)、国家重点研发计划项目(2021YFD2100401)、中央支持地方高校改革发展资金人才培养项目、黑龙江省优秀青年科学基金项目(YQ2022C021)和东北农业大学青年领军人才支持计划项目(NEAU2023QNLJ-007) （32202228）
