青岛大学学报（自然科学版）2024，Vol.37Issue(2)：22-28,7.DOI:10.3969/j.issn.1006-1037.2024.02.05
基于网络药理学和分子对接技术的2,3-吲哚醌抗少弱精症机制研究
Mechanistic Study of Isatin Against Oligoasthenozoospermia Based on Network Pharmacology and Molecular Docking
摘要
Abstract
To investigate the potential targets and mechanisms of isatin(ISA)in the treatment of oligoas-thenozoospermia,targets of ISA and disease-related targets were identified utilizing public databases to find intersecting targets.Core targets were then determined using Cytoscape software.GO functional en-richment and KEGG pathway analyses on the intersecting targets were conducted,and molecular docking was used to predict the binding affinity of ISA with core target proteins.The results indicate that ISA's in-tervention in oligoasthenozoospermia primarily involves biological processes such as oxidative stress,apop-tosis,and inflammation,and is closely related to the p53 signaling pathway,the cell senescence pathway,and the IL-17 signaling pathway.After screening,eight core targets were identified,with ISA stably bind-ing to six of them.Those suggest that ISA may exert its anti-oligoasthenospermia effects by modulating the p53 signaling pathway and the IL-17 signaling pathway through core targets including CASP3,TP53,ESR1,PTGS2,TNF and ANXA5.关键词
2,3-吲哚醌/网络药理学/少弱精症/分子对接Key words
isatin/network pharmacology/oligoasthenozoospermia/molecular docking分类
医药卫生引用本文复制引用
倪倍倍,代梦,毕晓林,赵志臣,岳旺,隋忠国..基于网络药理学和分子对接技术的2,3-吲哚醌抗少弱精症机制研究[J].青岛大学学报（自然科学版）,2024,37(2):22-28,7.基金项目
国家自然科学基金(批准号:30070867)资助. （批准号:30070867）
