食管疾病2024，Vol.6Issue(2)：130-133,4.DOI:10.15926/j.cnki.issn2096-7381.2024.02.008
牙龈卟啉单胞菌促进口腔鳞癌恶性演进
Porphyromonas gingivalis Enhances Malignant Progression of Oral Squamous Cell Carcinoma
摘要
Abstract
Objective To explore the cellular and epithelial-mesenchymal transition(EMT)-related mol-ecule expression changes as well as tumor-enhancing effects in oral squamous cell carcinoma cells(SCC-25)after Pg infection.Methods The abilities of cell proliferation,migration,invasion as well as subcutaneous tumor growth were evaluated after Pg infection of oral SCC-25 in the absence or presence of metronidazole.Immunohistochemistry and Western-blot were utilized to detect the protein levels of EMT-re-lated molecules.Results Pg infection promoted the proliferation,migration,and invasion as well as sub-cutaneous xenografted tumor growth of SCC-25 cells.Moreover,Pg also induced downregulation of E-cad-herin protein and upregulation of N-cadherin protein and Vimentin protein.Metronidazole treatment reversed the increased abilities of cell proliferation,migration,invasion,and protein expression of EMT-related mol-ecules induced by Pg infection.Conclusion Pg induces EMT and enhances the malignant progression in oral squamous cell carcinoma关键词
牙龈卟啉单胞菌/口腔鳞癌/裸鼠皮下荷瘤/上皮间质转化Key words
Porphyromonas gingivalis/oral squamous cell carcinoma/subcutaneous xenografted tumor/epithelial-mesenchymal transition分类
医药卫生引用本文复制引用
金丽佳,高鑫,杨付田,李伟,赵鹏飞,刘其伟..牙龈卟啉单胞菌促进口腔鳞癌恶性演进[J].食管疾病,2024,6(2):130-133,4.基金项目
河南省教育厅高校重点科研项目(24A310015)许昌市重点研发与推广专项科技攻关项目(20230213088) （24A310015）
