食管疾病2024，Vol.6Issue(2)：141-147,7.DOI:10.15926/j.cnki.issn2096-7381.2024.02.010
基于加权基因共表达网络鉴定食管癌浸润性淋巴细胞关键节点基因
Identification of Key Hub Genes in Infiltrating Lymphocytes of Esophageal Carcinoma Based on Weighted Gene Co-expression Network
摘要
Abstract
Objective To use Weighted Gene co-expression Network Analysis(WGCNA)identifing key genes associated with esophageal cancer and provide data support for related research,in order to iden-tify the gene expression of the infiltrating lymphocyte antigen receptor region in esophageal cancer.Meth-ods Tissue samples of 70 esophageal cancer patients from our hospital was selected and gene sequenced,meanwhile,transcriptome data were downloaded from NCBI SRA database.The co-expression network of e-sophageal cancer was constructed by R WGCNA,and the co-expression modules that most strongly correlated with esophageal squamous cell carcinoma were screened out.The hub genes were analyzed by Centiscape.Results The results of network analysis indicate that MEgrey60、MEpurple and MEblack modules were highly correlated with esophageal cancer.According to protein-protein interaction,the top 20 hub genes of each module were selected.The biological function of the genes in the module are mainly in-volved in endopeptidase inhibitor activity,specific RNA polymerase Ⅱ transcription factor activity,histone binding,antioxidant activity,thioredoxin peroxidase activity,ATPase activity and protein transporter activi-ty.Conclusion The key genes of infiltrating lymphocytes in esophageal cancer identified in this study can provide targets and theoretical basis for the diagnosis,treatment,and immunotherapy of esophageal cancer.关键词
食管癌/WGCNA/靶点/节点基因/肿瘤浸润淋巴细胞Key words
esophageal cancer/WGCNA/target/hub gene/tumor-infiltrating lymphocytes分类
医药卫生引用本文复制引用
侯永杰,曹恒,杨海军,周福有..基于加权基因共表达网络鉴定食管癌浸润性淋巴细胞关键节点基因[J].食管疾病,2024,6(2):141-147,7.基金项目
河南省科技攻关项目(212102310718)安阳市科技攻关项目(2022C01SF036) （212102310718）
