外源复合酶配比优化及其对犊牛饲粮体外发酵特性的影响OA北大核心CSTPCD
Optimization of exogenous complex enzyme ratio and its effect on in vitro fermentation characteristics of calf diet
试验旨在研究外源复合酶的最适配比,探索其对犊牛饲粮体外发酵特性的影响.采用正交试验设计模拟瘤胃发酵模型确定5种酶(植酸酶、淀粉酶、纤维素酶、果胶酶、蛋白酶)复配的最适比例,将筛选出的外源复合酶添加至犊牛饲粮中,模拟瘤胃环境进行体外试验,培养2、4、6、8、10、12、24、48 h,测定各时间点对照组和试验组的产气量(GP)、干物质降解率(DMD)及发酵参数.结果表明:5种酶的最佳添加量为植酸酶150 U/g、淀粉酶150 U/g、纤维素酶600 U/g、果胶酶1 200U/g、蛋白酶600 U/g,复合酶最适配比为150∶150∶900∶600∶600.与对照组相比,试验组的发酵液DMD、GP、氨态氮(NH3-N)、微生物蛋白(MCP)、总挥发性脂肪酸(TVFA)、乙酸、丙酸含量和乙丙比(A/P)均极显著提高(P<0.01).研究表明,饲粮中添加适宜配比的外源复合酶能够有效提高体外发酵GP、DMD,改善体外瘤胃发酵特性.
The purpose of this experiment was to study the optimum ratio of exogenous complex enzymes and explore their effects on the fermentation characteristics of calf diet in vitro.The optimum proportion of five enzymes(phytase,amylase,cellulase,pectinase,and protease)was determined by orthogonal design to simulate the rumen fermentation model.The exogenous complex enzymes were added to the diet of calves,and were cultured in vitro for 2,4,6,8,10,12,24,and 48 hours.The gas production(GP),dry matter degradation rate(DMD),and fermentation parameters of the control group and the experimental group were measured at each time point.The results showed that the optimum dosage of five enzymes was phytase 150 U/g,amylase 150 U/g,cellulase 600 U/g,pectinase 1 200 U/g,and protease 600 U/g,and the optimum ratio was 150∶150∶900∶600∶600.Compared with the control group,the contents of DMD,GP,ammonia nitrogen(NH3-N),microbial protein(MCP),total volatile fatty acid(TVFA),acetic acid,propionic acid,and the ratio of ethylene to propylene(A/P)in the fermentation broth of the experimental group were extremely increased(P<0.01).The study indicates that the addition of exogenous complex enzymes in diet can effectively increase the fermentation of GP and DMD in vitro and improve the rumen fermentation characteristics in vitro.
匡宇;马小雪;薛玉洋;牛玉杰;南珊珊;闫佳许;聂存喜
石河子大学动物科技学院,新疆石河子 832003
畜牧业
外源复合酶体外发酵瘤胃发酵参数犊牛
exogenous complex enzymein vitro fermentationrumen fermentation parameterscalf
《饲料研究》 2024 (011)
7-12 / 6
兵团重大科技项目(项目编号:2021AA004)
评论