上海针灸杂志2024，Vol.43Issue(6)：675-683,9.DOI:10.13460/j.issn.1005-0957.2024.06.0675
电针对干眼大鼠角膜自噬相关蛋白调节作用的研究
Study of electroacupuncture regulating autophagy-related proteins in the cornea of dry eye rats
摘要
Abstract
Objective To observe the effect of electroacupuncture in regulating autophagy in the cornea of dry eye rats.Method Male Sprague-Dawley(SD)rats were randomly divided into a normal group,a model group,an electroacupuncture group,and a sham acupuncture group.Except for the normal group,the other three groups received subcutaneous injection of Scopolamine solution plus exposure to air drought to establish dry eye rat models.After modeling,the electroacupuncture and sham acupuncture groups were given electroacupuncture or sham acupuncture,respectively.Before and after the intervention,tear film break-up time(BUT)and tear production were measured,and corneal fluorescein staining(CFS)was scored;rats'corneal ultrastructure was observed using transmission electron microscopy;Western blotting was adopted to determine the expression of microtubule-associated protein 1 light chain(LC3),P62,and Beclin-1 in the cornea;the real-time polymerase chain reaction(RT-PCR)technique was used to detect the expression of LC3 mRNA in rats'cornea.Result Compared with the normal group,the model group had a decreased tear production amount,shortened tear film BUT,and a higher CFS score,all showing statistical significance(P＜0.05);the model group also presented swollen corneal epithelial cells with significant damage to mitochondria,reduced protein expression of corneal LC3Ⅱ and Beclin-1,increased expression of P62 protein,and decreased expression of LC3 mRNA,and the differences were all statistically significant(P＜0.05).Compared with the model group,the electroacupuncture group had an increased tear secretion amount,extended tear film BUT,and a lower CFS score,all showing statistical significance(P＜0.05);in the electroacupuncture group,slightly swollen corneal epithelial cells were found,together with autophagosome formation,and the protein expression of corneal LC3Ⅱ and Beclin-1 increased,the P62 protein expression dropped,and the LC3 mRNA expression increased,all showing statistical significance(P＜0.05).Compared with the model group,the sham acupuncture group had significant organelle swelling and notable damage to mitochondria;there were no significant differences in the other indexes between these two groups(P＞0.05).Conclusion Electroacupuncture can encourage autophagy in the cornea in dry eye rats,which may be the potential mechanism of electroacupuncture in treating dry eye.关键词
针刺疗法/电针/干眼/角膜/自噬/大鼠Key words
Acupuncture therapy/Electroacupuncture/Dry eye/Cornea/Autophagy/Rats分类
中医学引用本文复制引用
杨延婷,刘婕,马晓芃,莫晓芬,马睿琦,杨光,师雅君,赵越,纪海洋,张丹,郭浩东..电针对干眼大鼠角膜自噬相关蛋白调节作用的研究[J].上海针灸杂志,2024,43(6):675-683,9.基金项目
国家自然科学基金项目(81904302) （81904302）
上海市自然科学基金项目(22ZR1458500,21ZR1460100) （22ZR1458500,21ZR1460100）
上海市卫生健康委员会项目(20214Y0116,20234Y0066) （20214Y0116,20234Y0066）
