Research on the Orientated Effective Components of Huangqi in Huangqi Jianzhong Tang Against Chronic Atrophic Gastritis Based on Multi-Spectrum-Effect CorrelationOACSTPCD

Objective:This study aimed to investigate the orientated effective components of Astragali Radix Huangqi(HQ)in HQ Jianzhong Tang(HQJZ),a classical formula of traditional Chinese medicine(TCM)used for treating chronic atrophic gastritis(CAG),using HQ as a monarch medicine.Materials and Methods:The spectra of HQJZ containing different polar parts of HQ were obtained using ultra-high-performance liquid chromatography-Q-Exactive mass spectrometry.Furthermore,the efficacy of HQJZ,which contains different polar parts of HQ,in treating rats with CAG was evaluated using traditional pharmacodynamic and nuclear magnetic resonance-based metabonomics.Grey relation analysis and partial least squares analysis were applied to analyze the spectrum-effect relationship and to screen out the orientated effective components related to HQ in the treatment of CAG.Results:Spectrum-effect relationship analysis showed that 24 compounds identified from the fingerprint spectrum were strongly correlated with efficacy.Compounds 8(calycosin-7-O-glc-6"-O-acetate),9(3-hydroxy-9,10-dimethoxyptercarpan),and 22(astragaloside II)were ranked among the top three.Conclusions:This study showed that integrating metabolomics and spectrum-effect relationship analysis is a powerful tool for obtaining orientated effective components of Chinese medicine in a given TCM formula.