生态学杂志2024，Vol.43Issue(6)：1691-1702,12.DOI:10.13292/j.1000-4890.202406.039
土壤微生物群落结构及碳循环功能对高寒草甸退化和人工建植的响应
Responses of soil microbial structure and carbon cycling functions to degradation and artificial restoration of alpine meadows
摘要
Abstract
We examined the impacts of degradation and artificial restoration of alpine grasslands on microbial com-munities and the related carbon cycling functions,with experiments being conducted on degraded alpine meadows and artificially restored grasslands in the Qinghai-Tibet Plateau.With metagenomic techniques,we analyzed the change of microbial structures and carbon cycling functions,and further explored the environmental factors driving the changes of carbon cycling functional genes.The results showed that degradation and artificial restoration of grasslands significantly altered soil and vegetation characteristics.The increase of soil microbial richness and diver-sity and the decrease of the relative abundance of dominant species indicated that microbial community tended to be homogeneous during degradation and artificial restoration of grasslands.The diversity of soil carbon cycling function-al genes showed a trend of increasing,stabilizing,and increasing.The top 15 dominant species contributed more than 40％to carbon cycle functional genes,and such contribution decreased significantly under degradation and restoration of grasslands.The effect of individual soil or vegetation on microbial community structure was greater than that on microbial carbon cycling functional genes,while soil and vegetation interaction had stronger effect on the latter than the former.Specifically,carbon cycling functional gene diversity and abundance were significantly related to soil available nitrogen(ammonium nitrogen and nitrate nitrogen)and vegetation factors(coverage,bio-mass,and diversity).Our results contribute to a better understanding of microbial mechanisms of carbon cycling during meadow degradation and artificial restoration.关键词
青藏高原/三江源/宏基因组/退化高寒草甸/人工建植/碳循环功能基因Key words
Qinghai-Tibet Plateau/Three-River Headwaters region/metagenomics/degraded alpine meadow/arti-ficial restoration/carbon cycling functional genes引用本文复制引用
黄鹏,丁明军,张华,徐欢,邹天娥,张月菊,曾欢..土壤微生物群落结构及碳循环功能对高寒草甸退化和人工建植的响应[J].生态学杂志,2024,43(6):1691-1702,12.基金项目
第二次青藏高原综合科学考察研究项目(2019QZKK0603)和国家自然科学基金项目(42061018)资助. （2019QZKK0603）
