不同道地产区黄芩的主要成分和抗氧化活性的比较研究OA北大核心CSTPCD
Comparative study on main components and antioxidant activity of Scutellaria Radix from different genuine producing areas
探究山东莱芜与河北承德两个道地产区黄芩的黄酮特征指纹图谱及其抗氧化能力的谱-效关系.在构建黄芩超高效液相色谱(ultra-performance liquid chromatography,UPLC)指纹图谱的基础上,采用体外抗氧化法测定25批不同道地产区黄芩对DPPH、ABTS、O2-自由基的清除能力,计算其半数抑制浓度(half maximal inhibitory concentration,IC50);运用正交偏最小二乘判别(orthogonal partial least-squares discrimination analysis,OPLS-DA)法和双变量相关性分析构建化学成分和抗氧化活性的谱-效关系.25批样品的指纹图谱相似度均大于0.983,确定了 16个共有峰,采用超高效液相色谱串联四级杆飞行时间质谱联用法(ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry,UPLC-Q-TOF-MS)确认了 12个化合物.两个道地产区的黄芩均具有较强的清除DPPH、ABTS和O2-自由基能力,其中山东莱芜黄芩的抗氧化能力优于河北承德黄芩.OPLS-DA所建的两个模型,均可成功区分两个道地产区的黄芩,且黄芩苷、黄芩素和千层纸素A-7-O-β-D-葡萄糖醛酸苷是二者的差异性标志成分.谱效分析发现,黄芩药材中黄酮类成分相互间可能对DPPH、ABTS、O2-自由基的清除能力具有协同增效作用.本研究建立了基于化学成分和抗氧化活性的黄芩谱效评价模式,可用于不同道地产区黄芩品质的评价,可为中药材的道地性研究提供帮助.
This study aims to explore the flavonoid characteristic fingerprint and antioxidant capacity spectrum-effect relation-ship of Scutellaria Radix in Laiwu,Shandong Province and Chengde,Hebei Province.On the basis of constructing ultra-high performance liquid chromatography(UPLC)fingerprint profiles of Scutellaria Radix,this study employed an in vitro antioxi-dant assay to evaluate the scavenging ability of 25 batches of Scutellaria Radix from different authentic production areas on DPPH,ABTS and O2-free radicals,and their half maximal inhibitory concentration(IC50)values were determined.Orthogonal partial least-squares discriminant analysis(OPLS-DA)method and bivariate correlation analysis were employed to establish the spectrum-efficacy relationship between the chemical composition and antioxidant activity.The fingerprint similarity of 25 batches samples exceeded 0.983,in which 16 common peaks were identified.Twelve compounds were identified by ultra-per-formance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS)technology.The Scutellaria Radix from the two areas had strong scavenging abilities of DPPH,ABTS and O2-free radicals,and the antioxidant capacity of Scutellaria Radix from Laiwu,Shandong province was superior to that from Chengde,Hebei Province.The two OPLS-DA mod-els can successfully distinguish Scutellaria Radix from two genuine producing areas.Baicalin,baicalein and oroxylin A-7-O-β-D-glucuronide were differential markers in them.The spectrum-efficacy analysis revealed that flavonoids in Scutellaria Radix may increase the scavenging abilities of DPPH,ABTS and O2-free radicals based on their synergistic effects.This study estab-lished the spectrum-efficacy evaluation model combined chemical composition with antioxidant activity of Scutellaria Radix.It can be used to evaluate the quality of Scutellaria Radix from different genuine producing areas,and can provide help for the reseach of the geotropism of Chinese medicinal materials.
刘雨然;郭梦佳;杨立新;瞿燕;潘晓丽;杨滨;李化
成都中医药大学药学院,成都 611137||中国中医科学院中药研究所,北京 100700中国中医科学院中药研究所,北京 100700成都中医药大学药学院,成都 611137
中医学
黄芩超高效液相色谱抗氧化指纹图谱谱效关系道地
Scutellaria RadixUPLCantioxidant activityfingerprintsspectrum-effect relationshipgeotropism
《天然产物研究与开发》 2024 (006)
919-929 / 11
山东省重点研发计划项目(2021CXGC010508);中国中医科学院科技创新工程项目(CI2021A04511);四川省科技厅项目(2023NSFSC0326)
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