灵芝多糖对糖尿病肾病小鼠的改善作用及机制研究OA北大核心CSTPCD
Study on the improvement effect and mechanism of Ganoderma lucidum polysaccharides on diabetic nephropathy in mice
研究了灵芝多糖(Ganoderma lucidum polysaccharide,GLP)对糖尿病肾病(diabetic nephropathy,DN)小鼠的作用.成功构建DN小鼠模型后GLP干预.检测小鼠空腹血糖和24 h尿蛋白含量,测定尿素氮(blood urea nitrogen,BUN)和肌酐(serum creatinine,Scr)水平,结合伊红和马松染色观察肾脏病理形态,采用酶联免疫吸附试验测定肿瘤坏死因子 α(tumor necrosis factor-alpha,TNF-α)、白细胞介素 6(interleukin-6,IL-6)和白细胞介素 10(interleukin-10,IL-10)的水平,使用蛋白免疫印迹法检测晚期糖基化终产品受体(receptor for advanced glycation end-products,RAGE)、Ⅳ型胶原蛋白(collagen type Ⅳ,COL-Ⅳ)和诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)蛋白的表达含量.DN组小鼠空腹血糖、24 h尿蛋白、BUN和Scr及其肾组织中TNF-α和IL-6水平均升高(P<0.05),血清中IL-10水平降低(P<0.05),RAGE、COL-Ⅳ和iNOS蛋白的表达量升高(P<0.05);而治疗组小鼠病理损伤及纤维化改变均有改善,定量指标得到好转.GLP可以改善DN小鼠,机制与抑制RAGE信号通路有关.
The study investigated the effects of Ganoderma lucidum polysaccharide(GLP)on diabetic nephropathy(DN)in mice.After successfully establishing a DN mouse model,GLP intervention was administered.The fasting blood glucose and 24-hour urinary protein content of the mice were measured,and the levels of blood urea nitrogen(BUN)and serum creatinine(Scr)were determined.Eosin and Masson's trichrome staining were used to observe the renal pathological morphology.En-zyme-linked immunosorbent assay was employed to measure the levels of tumor necrosis factor-alpha(TNF-α),interleukin-6(IL-6),and interleukin-10(IL-10).Western blot was utilized to detect the expression of receptor for advanced glycation end-products(RAGE),collagen type Ⅳ(COL-Ⅳ),and inducible nitric oxide synthase(iNOS).Mice in the DN group exhibited increased levels of fasting blood glucose,24-hour urinary protein,BUN,and Scr as well as elevated levels of TNF-α and IL-6 in renal tissue(P<0.05),and decreased levels of serum IL-10(P<0.05).The expression of RAGE,COL-Ⅳ,and iNOS proteins was also increased(P<0.05).In contrast,mice in the treatment group showed improvements in pathological dam-age and fibrosis,with quantitative indicators showing significant amelioration.GLP could ameliorate DN in mice,and the mechanism is associated with the inhibition of the RAGE signaling pathway.
马广礼;夏晓培
许昌职业技术学院医疗卫生学院,许昌 461000
中医学
灵芝多糖RAGE信号糖尿病肾病炎症
Ganoderma lucidum polysaccharideRAGE signalingdiabetic nephropathyinflammation
《天然产物研究与开发》 2024 (006)
938-945 / 8
河南省高等教育教学改革研究与实践项目(2019SJGLX716)
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