天津中医药2024，Vol.41Issue(6)：766-772,7.DOI:10.11656/j.issn.1672-1519.2024.06.16
肾炎防衰液对硫酸苯酯诱导小鼠肾足细胞损伤及自噬作用机制研究
Study on the mechanism of Shenyan Fangshuai Formula on induction mouse kidney podocytes injury and autophagy by phenyl sulfate
摘要
Abstract
[Objective]To observe the effect of Shenyan Fangshuai Formula on phenyl sulfate(PS)-induced mouse kidney podocyte injury and autophagy-related protein expression,and to explore its intervention effect on podocyte injury and autophagy.[Methods]We selected SD rats and gavaged Shenyan Fangshuai Formula and valsartan aqueous solution to prepare low,medium and high concentration drug-containing serum and western control serum,respectively.Podocytes were cultured in vitro and divided into normal control(Con)group,PS stimulation(PS)group,Shenyan Fangshuai Formula low,medium,and high three concentration treatment groups(SYFS-L,SYFS-M,and SYFS-H),and valsartan treatment(XST)group.The CCK-8 method screened the most appropriate intervention concentration and administration concentration of medicated serum.qRT-PCR and Western blot were used to detect Podocin,CD2AP,BNIP3,p62,and LC3Ⅱ/Ⅰ mRNA and protein expression.[Results]CCK-8 method to detect the cellular value-added rate identified 190 μmol/L concentration of PS to treat the podocytes for 24h as the modeling method for this experiment.Compared with the Con group,CD2AP,Podocin,BNIP3,LC3Ⅱ/Ⅰprotein and mRNA expression were significantly down-regulated and p62 protein and mRNA expression were significantly up-regulated in the PS group.Compared with the PS group,the Shenyan Fangshuai Formula group could significantly promote the expression of CD2AP,Podocin,BNIP3,LC3 mRNA and protein,and reduce the expression of p62,and it was better than that in the valsartan group.[Conclusion]Phenyl sulfate may cause podocyte injury by inhibiting podocyte mitochondrial autophagy,while Shenyan Fangshuai Formula ameliorates podocyte injury by up-regulating LC3,BNIP3,and down-regulating p62 protein expression,enhancing cellular autophagy,and inhibiting apoptosis.关键词
肾炎防衰液/硫酸苯酯/MPC5足细胞/自噬Key words
Shenyan Fangshuai Formula/phenyl sulfate/MPC5 podocyte/autophagy分类
医药卫生引用本文复制引用
张婧,马雷雷,杨思齐,韩玉,赵晰,王耀光..肾炎防衰液对硫酸苯酯诱导小鼠肾足细胞损伤及自噬作用机制研究[J].天津中医药,2024,41(6):766-772,7.基金项目
国家自然科学基金青年项目(82004316) （82004316）
天津市研究生科研创新项目(2022BKY193) （2022BKY193）
天津中医药大学研究生科研创新项目(YJSKC-20221013) （YJSKC-20221013）
国家中医药管理局:王耀光全国名老中医药专家传承工作室(978022) （978022）
天津市卫生健康委员会:中医药事业传承与发展:王耀光天津市名中医传承工作室(883022) （883022）
院级课题:基于深度学习的智能中医诊疗系统研究(63185021). （63185021）
