天然产物研究与开发2024，Vol.36Issue(6)：946-953,8.DOI:10.16333/j.1001-6880.2024.6.004
有氧运动和白藜芦醇对糖尿病肾病大鼠肾组织炎症和凋亡的影响
Effects of aerobic exercise and resveratrol on the inflammation and apoptosis in kidney tissue of diabetes rats
摘要
Abstract
The aim of this study was to investigate the effects of aerobic exercise and resveratrol on renal inflammation and ap-optosis in type 2 diabetic rats and its mechanism.Sixty-five SD rats were selected to establish diabetic rat model by high glu-cose and high fat feeding combined with chemical induction.The rats were randomly divided into diabetic group,exercise group,drug group and exercise drug group,and normal control group.Exercise groups completed eight consecutive weeks of treadmill running.Drug groups were given resveratrol liquid once a day for eight weeks.At the end of eight weeks,blood glu-cose,24 h urinary albumin(24 h-UP),serum creatinine(Scr)and blood urea nitrogen(BUN)were measured.The number of apoptosis in renal tissue was detected by TUNEL method.The expressions of interleukin-6(IL-6),tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β)were detected by ELISA.The expressions of apoptosis factors B cell lymphoma-2(Bcl-2),Bcl-2-associated X protein(Bax)and cysteinyl aspartate specific proteinase 3(Caspase-3)were detected by immunohis-tochemistry.The expression of NF-KBp65 in renal was detected by Western blot.Compared with normal control group,the lev-els of Scr,BUN,blood glucose,24 h urinary protein,inflammation-related factors IL-6,IL-1β and TNF-α in model group were increased(P＜0.05).Apoptosis of renal cells increased significantly,the expressions of Bax,Caspase-3 and NF-KBp65 in-creased significantly,and the expression of Bcl-2 decreased significantly(P＜0.05).The levels of Scr,BUN,blood glucose,24 h urinary protein,IL-6,IL-1β and TNF-α in exercise group,drug group and exercise drug group were decreased to different degrees(P＜0.05).The apoptosis of renal cells were decreased,the expressions of Bax,Caspase-3 and NF-KBp65 were sig-nificantly decreased(P＜0.05),and the expression of Bcl-2 was significantly increased(P＜0.05),and the changes were more obvious in the exercise drug group.The results indicated that aerobic exercise and resveratrol alone or combined inter-vention have a certain protective effect on diabetic nephropathy rats,and the mechanism may be related to inhibiting the acti-vation of NF-KBp65,reducing the level of inflammatory factors and inhibiting the apoptosis of kidney cells.Aerobic exercise combined with resveratrol intervention is better than single aerobic exercise or resveratrol intervention.关键词
2型糖尿病/有氧运动/白藜芦醇/炎症/细胞凋亡Key words
type 2 diabetes/aerobic exercise/resveratrol/inflammation/apoptosis分类
医药卫生引用本文复制引用
孙晓娟,吴安楠,侯娜,李娜..有氧运动和白藜芦醇对糖尿病肾病大鼠肾组织炎症和凋亡的影响[J].天然产物研究与开发,2024,36(6):946-953,8.基金项目
陕西省教育厅科学研究计划(20JK1936) （20JK1936）
