天然产物研究与开发2024，Vol.36Issue(6)：954-962,9.DOI:10.16333/j.1001-6880.2024.6.005
沙棘黄酮对长期力竭运动大鼠心肌保护作用的机制研究
Mechanism of sea-buckthorn flavone on myocardial protection in rats after long-term exhausted exercise
摘要
Abstract
The study was conducted to observe the protective effect of sea-buckthorn flavone(SF)on myocardium in rat sub-jected to long-term exhausted exercise.Fifty six-week-old SPF-grade male SD rat were randomly divided into normal group,model group,and SF low,medium and high dose(100,200 and 400 mg/kg)group.An exercise induced myocardial injury model was established through six-week exhausted treadmill training.Inflammatory reaction in myocardium,oxidative stress re-lated index and the serum markers of myocardial injury were detected by ELISA;myocardial cysteinyl aspartate specific pro-teinase-3(Caspase-3)protein expression was detected by the immunohistochemical method;protein expressions of myocardial BCL2-associated X(Bax),B-cell lymphoma-2(Bcl-2),type Ⅰ Collagen(Collagen-Ⅰ),and connective tissue growth factor(CTGF)were detected by Western blot;myocardial tissue morphology and collagen deposition were observed by HE and Mas-son staining.The results showed that compared with the model group,the levels of serum aspartate aminotransferase(AST),creatine kinase MB(CK-MB),cardiac troponin Ⅰ(cTn Ⅰ),collagen volume fraction(CVF),and the expression of Caspase-3,Bax,and Collagen-Ⅰ proteins in the myocardial tissue of rats in each dose group of SF were significantly reduced(P＜0.01),while the expression of Bcl-2 protein and Bcl-2/Bax ratio in the myocardial tissue were significantly increased(P＜0.01);Medium to high doses of SF can significantly reduce malondialdehyde(MDA),interleukin-1β(IL-1β),Interleukin-6(IL-6),tumor necrosis factor-α(TNF-α)and expression of CTGF protein in myocardial tissue(P＜0.01),increase the levels of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)in myocardial tissue(P＜0.01).The results of the study suggested that medium and high doses of SF could inhibit oxidative stress,inflammatory reaction,cell apoptosis,and fibrosis in the myocardial tissues of rat subjected to long-term exhausted exercise,and alleviate myocardial injury.It was found that the effect of a high dose was better.关键词
力竭运动/沙棘黄酮/心肌损伤/细胞凋亡/纤维化Key words
exhaustive exercise/sea-buckthorn flavone/myocardial injury/apoptosis/fibrosis分类
医药卫生引用本文复制引用
孔海军,谌晓安,李新龙..沙棘黄酮对长期力竭运动大鼠心肌保护作用的机制研究[J].天然产物研究与开发,2024,36(6):954-962,9.基金项目
新疆维吾尔自治区自然科学基金(Y2021S037) （Y2021S037）
新疆维吾尔自治区高校科技计划(XJEDU2021SY040) （XJEDU2021SY040）
喀什地区科技计划(KS2020024) （KS2020024）
