沙棘黄酮对长期力竭运动大鼠心肌保护作用的机制研究OA北大核心CSTPCD
Mechanism of sea-buckthorn flavone on myocardial protection in rats after long-term exhausted exercise
观察沙棘黄酮(sea-buckthorn flavone,SF)对长期力竭运动大鼠心肌组织的保护作用.50只6周龄SPF级雄性SD大鼠随机分为正常组、模型组和SF低中高剂量组(100、200、400 mg/kg).通过连续6周力竭跑台训练建立运动性心肌损伤模型.ELISA法检测心肌炎症反应、氧化应激相关指标及血清心肌损伤标志物;免疫组织化学法检测心肌半胱天冬酶3(cysteinyl aspartate specific proteinase-3,Caspase-3)蛋白表达;免疫印迹法检测心肌Bcl-2相关X蛋白(Bcl-2 associated X protein,Bax)、B 淋巴细胞瘤-2 基因(B cell iymphoma-2,Bcl-2)、Ⅰ 型胶原(type Ⅰ Collagen,Colla-gen-Ⅰ)、结缔组织生长因子(connective tissue growth factor,CTGF)蛋白表达;HE、Masson染色观察心肌组织形态及胶原沉积.结果表明,与模型组比较,SF各剂量组大鼠血清谷草转氨酶(aspartate aminotransferase,AST)、肌酸激酶同工酶 MB(creatine kinase MB,CK-MB)、心肌肌钙蛋白 Ⅰ(cardiac troponin Ⅰ,cTn Ⅰ)、心肌组织胶原容积(collagen volume fraction,CVF)水平和心肌组织Caspase-3、Bax、Collagen-Ⅰ蛋白表达显著降低(P＜0.01),心肌组织Bcl-2蛋白表达和Bcl-2/Bax比值显著上升(P＜0.01);中高剂量SF还可显著降低大鼠心肌组织丙二醛(malondialdehyde,MDA)、白介素 1-β(interleukin-1β,IL-1β)、白介素-6(interleukin-6,IL-6)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)水平和心肌组织CTGF蛋白表达(P＜0.01),提高心肌组织超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)水平(P＜0.01).研究结果提示,中、高剂量SF可抑制长期力竭训练大鼠心肌组织氧化应激、炎症反应、细胞凋亡和纤维化,减轻心肌损伤,且高剂量作用效果更佳.
The study was conducted to observe the protective effect of sea-buckthorn flavone(SF)on myocardium in rat sub-jected to long-term exhausted exercise.Fifty six-week-old SPF-grade male SD rat were randomly divided into normal group,model group,and SF low,medium and high dose(100,200 and 400 mg/kg)group.An exercise induced myocardial injury model was established through six-week exhausted treadmill training.Inflammatory reaction in myocardium,oxidative stress re-lated index and the serum markers of myocardial injury were detected by ELISA;myocardial cysteinyl aspartate specific pro-teinase-3(Caspase-3)protein expression was detected by the immunohistochemical method;protein expressions of myocardial BCL2-associated X(Bax),B-cell lymphoma-2(Bcl-2),type Ⅰ Collagen(Collagen-Ⅰ),and connective tissue growth factor(CTGF)were detected by Western blot;myocardial tissue morphology and collagen deposition were observed by HE and Mas-son staining.The results showed that compared with the model group,the levels of serum aspartate aminotransferase(AST),creatine kinase MB(CK-MB),cardiac troponin Ⅰ(cTn Ⅰ),collagen volume fraction(CVF),and the expression of Caspase-3,Bax,and Collagen-Ⅰ proteins in the myocardial tissue of rats in each dose group of SF were significantly reduced(P＜0.01),while the expression of Bcl-2 protein and Bcl-2/Bax ratio in the myocardial tissue were significantly increased(P＜0.01);Medium to high doses of SF can significantly reduce malondialdehyde(MDA),interleukin-1β(IL-1β),Interleukin-6(IL-6),tumor necrosis factor-α(TNF-α)and expression of CTGF protein in myocardial tissue(P＜0.01),increase the levels of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)in myocardial tissue(P＜0.01).The results of the study suggested that medium and high doses of SF could inhibit oxidative stress,inflammatory reaction,cell apoptosis,and fibrosis in the myocardial tissues of rat subjected to long-term exhausted exercise,and alleviate myocardial injury.It was found that the effect of a high dose was better.
孔海军;谌晓安;李新龙
喀什大学体育学院,喀什 844000||吉首大学体育科学学院,湘西 416000吉首大学体育科学学院,湘西 416000喀什大学体育学院,喀什 844000
特种医学
力竭运动沙棘黄酮心肌损伤细胞凋亡纤维化
exhaustive exercisesea-buckthorn flavonemyocardial injuryapoptosisfibrosis
《天然产物研究与开发》 2024 (006)
954-962 / 9
新疆维吾尔自治区自然科学基金(Y2021S037);新疆维吾尔自治区高校科技计划(XJEDU2021SY040);喀什地区科技计划(KS2020024)
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