天津中医药2024，Vol.41Issue(6)：773-780,8.DOI:10.11656/j.issn.1672-1519.2024.06.17
清肝活血方调控Caspase-4/Caspase-3/GSDME介导的细胞焦亡改善酒精性肝损伤
Qinggan Huoxue Recipe regulates the cell pyroptosis mediated by Caspase-4/Caspase-3/GSDME to improve alcoholic liver injury
摘要
Abstract
[Objective]To investigate the mechanism of Qinggan Huoxue Recipe(Qinggan Huoxue Recipe,QGHXR)in the prevention and treatment of alcoholic liver disease(ALD)by regulating Caspase-4/Caspase-3/GSDME pyroptosis pathway.[Methods]Eighteen C57BL/6 mice were randomly divided into control group,model group and QGHXR group to replicate the alcoholic liver disease model.Serum liver function and lipid levels were detected.Hematoxylin-eosin(HE)staining and oil red O staining were used to observe the pathological changes and lipid deposition in the liver.The expression of Caspase-4/Caspase-3/GSDME pathway related genes and proteins were detected by q-RT PCR,Western Blot and immunofluorescence staining.[Results]Compared with the control group,the liver TG level in model group was significantly increased(P<0.01),the serum ALT and AST levels were increased(P<0.05),and the serum IL-1β,IL-6 and TNF-α levels were increased(P<0.05).Liver tissue showed pathological changes and lipid deposition.The mRNA expression levels of IL-1β,IL-6,TNF-α and NLRP3 in liver tissue were increased.Compared with model group,the liver TG level in QGHXR group was significantly decreased(P<0.01),and the serum ALT,AST,TBIL and TBA levels were decreased(P<0.05).Serum TG,IL-6 and TNF-α levels were decreased(P<0.05)and serum HDL levels were increased(P<0.01).Serum lipid,liver pathological changes and lipid deposition were relieved.The levels of GsdmeN,Caspase-4,Caspase-3 and C-Caspase-3 genes and proteins were decreased(P<0.05).[Conclusion]QGHXR alleviates liver damage and lipid deposition in ALD mice by modulating the Caspase-4/Caspase-3/GSDME-mediated pyroptosis pathway.关键词
清肝活血方/酒精性肝病/焦亡/Caspase-4/Caspase-3/GSDME通路/中药复方Key words
Qinggan Huoxue Recipe/alcoholic liver disease/pyroptosis/Caspase-4/Caspase-3/GSDME pathway/traditional Chinese medicine compound分类
医药卫生引用本文复制引用
吴琰,圣炜,王立顺,张莉,计康,陈绘佳,陈珺明..清肝活血方调控Caspase-4/Caspase-3/GSDME介导的细胞焦亡改善酒精性肝损伤[J].天津中医药,2024,41(6):773-780,8.基金项目
上海市闵行区中医特色品牌专科(ZYPP-02),上海市卫生健康委员会中医药科研项目(2022QN073). （ZYPP-02）
