清肝活血方调控Caspase-4/Caspase-3/GSDME介导的细胞焦亡改善酒精性肝损伤OACSTPCD

[Objective]To investigate the mechanism of Qinggan Huoxue Recipe(Qinggan Huoxue Recipe,QGHXR)in the prevention and treatment of alcoholic liver disease(ALD)by regulating Caspase-4/Caspase-3/GSDME pyroptosis pathway.[Methods]Eighteen C57BL/6 mice were randomly divided into control group,model group and QGHXR group to replicate the alcoholic liver disease model.Serum liver function and lipid levels were detected.Hematoxylin-eosin(HE)staining and oil red O staining were used to observe the pathological changes and lipid deposition in the liver.The expression of Caspase-4/Caspase-3/GSDME pathway related genes and proteins were detected by q-RT PCR,Western Blot and immunofluorescence staining.[Results]Compared with the control group,the liver TG level in model group was significantly increased(P<0.01),the serum ALT and AST levels were increased(P<0.05),and the serum IL-1β,IL-6 and TNF-α levels were increased(P<0.05).Liver tissue showed pathological changes and lipid deposition.The mRNA expression levels of IL-1β,IL-6,TNF-α and NLRP3 in liver tissue were increased.Compared with model group,the liver TG level in QGHXR group was significantly decreased(P<0.01),and the serum ALT,AST,TBIL and TBA levels were decreased(P<0.05).Serum TG,IL-6 and TNF-α levels were decreased(P<0.05)and serum HDL levels were increased(P<0.01).Serum lipid,liver pathological changes and lipid deposition were relieved.The levels of GsdmeN,Caspase-4,Caspase-3 and C-Caspase-3 genes and proteins were decreased(P<0.05).[Conclusion]QGHXR alleviates liver damage and lipid deposition in ALD mice by modulating the Caspase-4/Caspase-3/GSDME-mediated pyroptosis pathway.