天津中医药2024，Vol.41Issue(6)：789-794,6.DOI:10.11656/j.issn.1672-1519.2024.06.19
基于Box-Behnken响应面设计优选中药复方便乃通的提取工艺
Optimization of the extraction process for Chinese herbal compound Biannaitong based on Box-Behnken response surface design
殷倩倩 1李亦武 2邓秀平 1钟雨虹 1李鑫 1李小英 2刘锴 2李嘉瑞 2付茉莉 2刘志东1
作者信息
- 1. 天津中医药大学组分中药国家重点实验室,天津 301617||天津中医药大学现代中药发现与制剂技术教育部工程研究中心,天津 301617
- 2. 武汉同济现代医药科技股份有限公司,武汉 430056
- 折叠
摘要
Abstract
[Objective]To optimize the extraction process of Chinese medicine compound Biannaitong(BNT)by Box-Behnken response surface design.[Methods]Based on the single factor test,the influencing factors and range of BNT extraction process were optimized.The three-factor and three-level test design scheme was constructed by Box-Behnken response surface design method.The content of sennoside B,sennoside A and hesperidin was used as the response index to optimize the best extraction process conditions,and the process verification was carried out.[Results]The optimum extraction conditions of Chinese herbal compound BNT were as follows:solid-liquid ratio of 1∶8,reflux time of 30 min,extracting for 2 times.[Conclusion]The Box-Behnken response surface design method was used to optimize the BNT extraction process of traditional Chinese medicine compound.The method is stable and can provide a reference for the BNT extraction process.关键词
响应面设计/提取工艺/HPLCKey words
response surface design/extraction process/HPLC分类
医药卫生引用本文复制引用
殷倩倩,李亦武,邓秀平,钟雨虹,李鑫,李小英,刘锴,李嘉瑞,付茉莉,刘志东..基于Box-Behnken响应面设计优选中药复方便乃通的提取工艺[J].天津中医药,2024,41(6):789-794,6.
