怀山药内生真菌Chaetomium sp.的次生代谢产物研究OA北大核心CSTPCD
Secondary metabolites from the endophytic Chaetomium sp.of Dioscorea opposita
以一株怀山药内生毛壳菌Chaetomium sp.L-45为研究对象,对其次生代谢产物进行分离和结构鉴定.对毛壳菌进行大米发酵,通过硅胶柱色谱、Sephadex LH-20、半制备高效液相色谱等分离技术分离发酵物的乙酸乙酯提取物,利用NMR、HR-ESI-MS等波谱解析技术进行化合物的结构鉴定.从目标菌株的乙酸乙酯提取物中分离得到4个化合物,分别鉴定为3,4-二甲氧基-5-羟基-6-丙基-苯甲酸(1)、5-异戊烯基-吲哚-3-羧酸(2)、lecanorin(3)和麦角甾醇(4),其中,化合物1为新化合物,化合物2和3系首次从毛壳菌属真菌中分离得到.
In this study,the secondary metabolites of an endophytic Chaetomium sp.L-45 from Dioscorea opposita were isola-ted and identified.The ethyl acetate extract of the rice fermentation of the fungus were separated by silica gel column chroma-tography,Sephadex LH-20,semi-preparative High-performance liquid chromatography,etc.,and the chemical structures of the compounds were identified by NMR and HR-ESI-MS analysis.As a result,four compounds were isolated and identified as 3,4-dimethoxy-5-hydroxy-6-propyl-benzoic acid(1),5-isoprenylindole-3-carboxylic acid(2),lecanorin(3),and ergosterol(4).Among them,compound 1 is a new compound,compounds 2 and 3 were isolated from genus Chaetomium for the first time.
盛蒙珂;赵莹菲;田若丹;文春南;刘淼;麻兵继
河南农业大学农学院河南农业大学农学院||郑州市中药材种质资源创新与代谢工程重点实验室,郑州 450046
中医学
毛壳菌次生代谢产物提取分离结构鉴定
Chaetomium sp.secondary metabolitesextraction and isolationstructure elucidation
《天然产物研究与开发》 2024 (006)
982-985,962 / 5
国家自然科学基金(U1604169);河南省自然科学基金(232300421127);河南农业大学博士启动基金(30500662)
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