基于HPLC指纹图谱结合化学模式识别法对黄芪不同炮制品多指标成分分析研究OA北大核心CSTPCD

This study provides theoretical support for the comprehensive utilization of Astragali Radix processed products by establishing HPLC fingerprints and multi-component content determination methods for different processed products of Astra-gali Radix,and combining them with chemometric methods for comparative analysis.The fingerprints of Astragali Radix pro-cessed products were established by HPLC and the contents of six components were determined,and evaluate the differences of processed products of Astragali Radix by hierarchical clustering analysis(HC A),principal component analysis(PC A)and orthogonal partial least squares discriminant analysis(OPLS-DA)to find the differential components.The results showed that a total of 20 common peaks were obtained from different processed products(ten batches each),among which six common peaks were identified,and the linearity of the six components was good in their respective mass concentration ranges(r＞0.999),with average spiked recoveries ranging from 100.5％to 102.1％,and the relative standard deviation(RSD)was 0.77％-2.1％;The similarity results of fingerprint mapping showed that the similarity of ten batches each of honey bran-pro-cessed Astragali Radix,honey-processed Astragali Radix,salt-processed Astragali Radix,and wine-processed Astragali Radix was above 0.900,but the similarity of different types of concoctions decreased;Hierarchical clustering analysis can cluster the four kinds of processed products into three categories,the results of principal component analysis and cluster analysis are con-sistent and can be corroborated with each other,orthogonal partial least squares-discriminant analysis can effectively differen-tiate the four kinds of processed products,and nine main difference components were screened based on the variable impor-tance of projection(VIP)greater than 1,calycosin-7-glucoside,ononin,and 5-hydroxymethylfurfural were identified as the major landmark components causing compositional differences among different processed products of Astragali Radix.The HPLC fingerprint combined with stoichiometry of Astragali Radix processed products established by this institute is accurate and reliable,which can provide a reference for the quality control and quality evaluation of Astragali Radix processed prod-ucts.