天然产物研究与开发2024，Vol.36Issue(6)：1013-1021,9.DOI:10.16333/j.1001-6880.2024.6.012
19种中药提取物对海洋致病溶藻弧菌生长和生物被膜的影响
Effects of 19 Chinese herbal medicines extracts on the growth and biofilm formation of Vibrio alginolyticus
摘要
Abstract
;The outbreak and prevalence of Vibrio alginolyticus caused huge economic losses on marine aquaculture,with the formation of biofilms exacerbating the pathogenicity and antibiotic resistance.At present,Chinese herbal medicines has shown positive potential in the prevention and treatment of pathogenic bacterial infections.To further explore this potential,a study was conducted which 19 Chinese herbal medicines(Lycii Fructus,Paridis Rhizoma,Asteris Radix et Rhizoma,Polygoni Mul Tiflori Radix,Spatholobi Caulis,Magnoliae Officinalis Cortex,Ecliptae Herba,Desmodii StyracifolⅡ Herba,Gardeniae Fruc-tus,Downy Rosemyrtle,Forsythiae Fructus,Caryophylli Flos,Solani Lyrati Herba,Phellodendri Chinensis Cortex,Artemisiae Argyi Folium,Anemarrhenae Rhizoma,salt processed Anemarrhenae Rhizoma,wine processed Anemarrhenae Rhizoma,stir-frying processed Anemarrhenae Rhizoma)were carefully selected and subjected to extraction using various solvents to explore the effects of these extracts on the growth and biofilm formation of V.alginolyticus.The effects of these extracts on the growth of V.alginolyticus were systematically assessed through the utilization of the Oxford cup method and the 96-well plate method.Additionally,the potential influence of sub-inhibitory concentrations of these herbal extracts on biofilm formation of V.algino-lyticus was investigated by means of crystal violet staining.The findings indicated that water extracts of 12 Chinese herbal medicines,alcoholic extracts of 17 Chinese herbal medicines and ester extracts of 15 Chinese herbal medicines displayed noteworthy antibacterial activity.Significantly,the water extracts of Caryophylli Flos,alcoholic extracts of Paridis Rhizoma and Anemarrhenae Rhizoma,and ester extracts of Downy Rosemyrtle and Forsythiae Fructus demonstrated noteworthy inhibitory effects on the formation of biofilm by V.alginolyticus.Furthermore,distinctions in antibacterial activity were discerned between the unprocessed and processed forms of Anemarrhenae Rhizoma,despite their some similar impacts on biofilm formation.The results of this investigation carry substantial guiding significance for the prevention and management of bacterial infections and the development of agents that counteract biofilm formation.These findings are expected to provide new and sustainable strate-gies for responding to outbreaks of V.alginolyticus,helping to protect the sustainability of marine aquaculture,safeguard food safety,and safeguard human health.关键词
溶藻弧菌/中药提取物/生物被膜/抑菌Key words
Vibrio alginolyticus/Chinese herbal medicines extracts/biofilm/antibacterial分类
轻工纺织引用本文复制引用
李百元,张叶敏,夏晶晶,姜红宇,曹林艳,陈丹..19种中药提取物对海洋致病溶藻弧菌生长和生物被膜的影响[J].天然产物研究与开发,2024,36(6):1013-1021,9.基金项目
湖南科技学院科学研究一般项目(23XKYZZ03) （23XKYZZ03）
广东省海洋药物重点实验室开放项目(LMM2020-1) （LMM2020-1）
湖南省科技创新计划(2022RC1169) （2022RC1169）
国家自然科学基金青年基金(32100151) （32100151）
