天然产物研究与开发2024，Vol.36Issue(6)：1022-1031,10.DOI:10.16333/j.1001-6880.2024.6.013
基于特征图谱及多成分定量的首乌藤饮片与标准汤剂量值传递规律研究
Study on the quantity value transfer of Polygoni Multiflori Caulis decoction pieces to standard decoction based on fingerprint and multi-component quantification
摘要
Abstract
The content determination method and fingerprint evaluation method of Polygoni Multiflori Caulis were established.The quantity value transfer rule from Polygoni Multiflori Caulis(PMC)decoction pieces to PMC the standard decoction of Chinese materia medica was studied.Twenty batches of PMC from the main producing areas and authentic producing areas in China were collected.Prepared into decoction pieces of PMC in accordance with the provisions of the 2020 edition of Chinese Pharmacopoeia,and the standard decoction of Shouwuteng was prepared according to"Technical Requirements for Quality Control and Standard Formulation of Chinese Medicine Formula Granules".HPLC was used to establish the content determi-nation method and fingerprint method.The transfer rate,extraction rate and similarity of fingerprint were taken as indexes,the quantity value transfer rule from PMC decoction pieces to PMC standard decoction was analyzed.Results showed that the es-tablished HPLC method and fingerprint method were suitable for the determination of PMC decoction pieces and its standard decoction.The results showed that the paste rate of 20 batches of PMC standard decoction was 10.4％-16.1％,the mean val-ue was 12.9％,all were within±30％of the mean value.In addition to the samples of Sichuan origin,The HPLC fingerprint similarity of other origin of PMC decoction pieces and PMC standard decoction of Chinese materia medica were all good(＞0.9),And they have 6 peaks in common,Four of them were identified as 2,3,5,4-tetrahydroxystilbene-2-0-β-D-glucoside,emodin-8-O-β-D-glucoside,emodin,emodin-3-methyl ether.The transfer rates of the four known components were 26.58％-53.07％(2,3,5,4-tetrahydroxystilbene-2-0-β-D-glucoside),12.37％-42.84％(emodin-8-O-β-D-glucoside),3.83％-18.71％(emodin)and 0.35％-4.13％(emodin-3-methyl ether).Therefore,a method for the content and fingerprint of PMC and its standard decoction have been established,and the quantity value transfer law of chemical composition between them was expounded.The reference was provided for the quality control of water extract and formula granule of PMC.关键词
首乌藤/标准汤剂/转移率/指纹图谱/量值传递Key words
Polygoni Multiflori Caulis/standard decoction/transfer rate/fingerprint/quantity value transfer分类
医药卫生引用本文复制引用
曹斯琼,陈康梅,潘礼业,周湘媛,马懿飞..基于特征图谱及多成分定量的首乌藤饮片与标准汤剂量值传递规律研究[J].天然产物研究与开发,2024,36(6):1022-1031,10.基金项目
国家工业和信息化部2019年产业技术基础公共服务平台项目(2019-00902-1-2) （2019-00902-1-2）
