天然产物研究与开发2024，Vol.36Issue(6)：1056-1063,1055,9.DOI:10.16333/j.1001-6880.2024.6.016
基于网络药理学探究补肺活血胶囊治疗肺纤维化的潜在作用机制
Potential mechanism of Bufei Huoxue capsule in treating pulmonary fibrosis based on network pharmacology
摘要
Abstract
;To explore the potential mechanism and targets of Bufei Huoxue capsule(BHC)in anti-pulmonary fibrosis(PF).Firstly,TCMSP,SwissTargetPrediction and SEA databases were used to screen the active components and potential targets of BHC.GeneCards Suite,CTD and TTD databases were used to retrieve the disease-related targets of PF.The disease-related targets were mapped to the potential targets of the compound to obtain the public targets,which were then imported into the STRING platform for topological analysis with Cytoscape software to obtain the key targets.KEGG enrichment analysis was performed through the DAVID platform.To verify the data,Masson staining and chest CT were used to clarify the collagen deposition and imaging of lung tissue,and Western blot and q-PCR experiments were used to clarify the protein expression and gene transcription of the relevant targets.A total of 206 potential targets of BHC,487 targets of PF and 57 public targets were obtained.A total of 15 key targets,including TNF-α,MMP9 and p38,were screened out.Enrichment analysis suggested that TNF signaling pathway was the key pathway.Animal experiments showed that BHC could alleviate collagen deposition in lung tissue,reduce the fibrous grid shadow near the pleural tissue of mice lung tissue,and reduce the protein and mRNA con-tents of MMP9,TNF and p38 in lung tissue of PF mice(P＜0.01).This research reveals the mechanism of action of BHC in regulating PF through multiple components,multiple targets,and multiple pathways,which provides theoretical basis and sci-entific basis for the clinical application of BHC.关键词
补肺活血胶囊/肺纤维化/网络药理学/TNF信号通路Key words
Bufei Huoxue capsule/pulmonary fibrosis/network pharmacology/TNF signaling pathway分类
医药卫生引用本文复制引用
彭文潘,王单,武琦,徐泳,杭文璐..基于网络药理学探究补肺活血胶囊治疗肺纤维化的潜在作用机制[J].天然产物研究与开发,2024,36(6):1056-1063,1055,9.基金项目
江苏省自然科学基金青年项目(BK20220236) （BK20220236）
江苏省卫生健康委科研项目(Z2023023) （Z2023023）
江苏省职业健康科研项目(JSZJ20233202) （JSZJ20233202）
苏州市姑苏卫生人才计划(GSWS2022080) （GSWS2022080）
