天津科技大学学报2024，Vol.39Issue(3)：9-14,63,7.DOI:10.13364/j.issn.1672-6510.20230116
甲醛对甲醛裂合酶结构的修饰作用研究
Investigation of the Effect of Formaldehyde on the Structure of Formolase
摘要
Abstract
To investigate the effect of formaldehyde on formolase and the cause of its inactivation,in our present study for-molase was incubated with different concentrations of formaldehyde(300 mmol/L,1 mol/L)for different time(1 hour,2 hours and 3 hours).Subsequently,the residual activity of formolase was detected and the modified and cross-linked sites of formolase were detected by liquid chromatography-tandem mass spectrometry(LC-MS/MS).The results showed that there was a significant cross-linking and modification phenomenon of formolase after incubation with formaldehyde,and the higher of the formaldehyde concentration and the longer the incubation time,the higher degree of cross-linking of formolase.Two formaldehyde modification sites(N186 and K228)of formolase and potential formaldehyde cross-linked fragments were identified with LC-MS/MS.Through the cross-linking reaction with such amino acids as asparagine and lysine,formaldehyde destroyed its local structure and changed the overall aggregation state,thus making it inactive.This finding might be also beneficial for providing semi-rational design sites for improving formaldehyde tolerance of formolase.关键词
甲醛/甲醛裂合酶/甲醛耐受性/液相色谱-串联质谱Key words
formaldehyde/formolase/formaldehyde resistance/LC-MS/MS分类
生物科学引用本文复制引用
韩庚,张翠英,朱蕾蕾..甲醛对甲醛裂合酶结构的修饰作用研究[J].天津科技大学学报,2024,39(3):9-14,63,7.基金项目
国家重点研发计划项目(2022YFC2106000) （2022YFC2106000）
