世界中医药2024，Vol.19Issue(8)：1069-1073,5.DOI:10.3969/j.issn.1673-7202.2024.08.002
健脾温肾方对荷肉瘤小鼠腹水模型免疫细胞相关因子影响研究
Effects of Jianpi Wenshen Formula on Immune Cell-related Factors in the Ascites Model of Sarcoma-bearing Mice
摘要
Abstract
Objective:To explore the effects of Jianpi Wenshen Formula(JPWSF)on the levels of immune cell-related factors in the ascites model of sarcoma-180(SI 80)-bearing mice.Methods:Sixty C57 mice of 5 to 6 weeks old were randomized into a model group,a cisplatin group,a cisplatin+low-dose(9.75 g/kg)JPWSF group,and a cisplatin+high-dose(19.5 g/kg)JPWSF group,with 15 mice in each group.A ascites model of S180-bearing mice was established.The mice were administrated with cisplatin at a dose of 0.2 mg/kg by gavage on days 1,4,and 7 of each week for four weeks.JPWSF was administrated at corresponding doses once a day for a total of 4 weeks.The model group received intraperitoneal injection of an equal dose of normal saline on days 1,4,and 7 of each week for 4 weeks.The tumor weight,tumor inhibition rate,ascites liquid volume,red blood cell and tumor cell counts,carcino-embryonic antigen(CEA)level,and levels of immune cell-related factor were compared between groups.The survival time of the mice was recorded.Results:Compared with the cisplatin group,the additional administration of JPWSF increased the tumor inhibition rate in a dose-dependent manner(P＜0.05).Compared with the model group,the cisplatin,cisplatin+low-dose JPWSF,and cisplatin+high-dose JPWSF groups showed decreased tumor weight,ascites liquid volume,red blood cell count,and tumor cell count,lowered levels of CEA,vascular endothelial growth factor(VEGF),and interleukin-4(1L-4),elevated level of interferon-gam-ma(IFN-γ),and prolonged the survival time(P＜0.05).Compared with the cisplatin group,the additional administration of JP-WSF reduced the tumor weight,ascites liquid volume,red blood cell count,and tumor cell count,lowered the levels of CEA,VEGF,and IL-4,elevated the IFN-γ level,and prolonged the survival time in a dose-dependent manner(P＜0.05).Conclusion:JPWSF significantly inhibited the tumor,reduced the ascites liquid,lowered the VEGF and IL-4 levels,and elevated the IFN-γ level in the ascites model of S180-bearing mice.关键词
顺铂/健脾温肾方/荷S180肉瘤小鼠/腹水模型/免疫细胞相关因子/血管内皮生长因子/γ干扰素/白细胞介素-4Key words
Cisplatin/Jianpi Wenshen Formula/S180-bearing mice/Ascites model/Immune cell-related factors/Vascular endo-thelial growth factor/Interferon-gamma/Interleukin-4分类
医药卫生引用本文复制引用
黎智燊,张芸,罗智杰,史清华..健脾温肾方对荷肉瘤小鼠腹水模型免疫细胞相关因子影响研究[J].世界中医药,2024,19(8):1069-1073,5.基金项目
国家自然科学基金项目(81974571) （81974571）
广东省中医药局科研项目(20212010) （20212010）
