世界中医药2024，Vol.19Issue(8)：1085-1091,7.DOI:10.3969/j.issn.1673-7202.2024.08.005
基于肠道炎症反应研究降糖3号方抗糖尿病的作用机制
Anti-diabetic Mechanism of Jiang Tang San Hao Formula Based on Intestinal Inflammation
摘要
Abstract
Objective:To observe the effect of Jiang Tang San Hao Formula(JTSHF)on intestinal inflammation in the mouse mod-el of type 2 diabetic mellitus(T2DM)and reveal the anti-diabetic mechanism of JTSHF.Methods:Four-week-old C57BL/6N male mice were selected for the modeling of T2DM by a high-fat diet combined with injection of low-dose streptozotocin(STZ).The mod-eled mice were then randomized into a model group,a metformin group,and a JTSHF group,with 8 mice in each group.Eight mice were fed with a standard diet and served as the normal group.All the mice were administrated with corresponding drugs for 8 weeks.The cytokine antibody array kit was used to determine the levels of multiple inflammatory mediators in the colon tissue of mice.The differentially expressed proteins were screened,which was followed by Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment.Western blotting was employed to determine the activation of nucleotide-binding oligomerization domain leucine-rich repeat and pyrin domain-containing protein 3(NLRP3)inflammasome and the expression levels zonula occludens-1(ZO-1),occludin,G protein-coupled receptor 43(GPR43),apoptosis-associated speck-like protein containing a CARD(ASC),and caspase-1 in the colon tissue of mice.Results:The levels of inflammatory mediators in the colon tissue differed significantly between groups.Compared with the model group,the JTSHF group showed lowered levels of interleukin-1β(IL-1β)and IL-6,up-regulated expression of ZO-1,occludin,and GPR43,and down-regulated expression of ASC and caspase-1(P＜0.05).Conclusion:Obvious inflammation was observed in the colon tissue of T2DM mice,which was reduced by JTSHF.JTSHF may regulate the NLRP3 inflammasome and its downstream inflammatory mediators to exert the therapeutic effect.关键词
糖尿病/降糖3号方/炎症介质/核苷酸结合寡聚化结构域样受体蛋白3炎症小体/抗体芯片/结肠组织/作用机制Key words
Type 2 diabetes mellitus/Jiang Tang San Hao Formula/Inflammatory mediators/Nucleotide-binding oligomerization do-main leucine-rich repeat and pyrin domain-containing protein 3(NLPR3)inflammasome/Antibody array/Colon tissue/Mechanism分类
医药卫生引用本文复制引用
赵祎,徐冰蕊,叶紫梦玮,莫芳芳,穆倩倩,田甜,杨晓达,高思华,赵丹丹..基于肠道炎症反应研究降糖3号方抗糖尿病的作用机制[J].世界中医药,2024,19(8):1085-1091,7.基金项目
国家自然科学基金项目(82174329) （82174329）
国家中医药领军人才支撑计划-岐黄学者项目(1040063321005) （1040063321005）
北京中医药大学基本科研业务费揭榜挂帅项目(2023-JYB-JBZD-010) （2023-JYB-JBZD-010）
北京市中医药管理局科技发展基金项目(JJ2018-72&JJ2018-72) （JJ2018-72&JJ2018-72）
