武警医学2024，Vol.35Issue(6)：461-466,6.
ABCA1 DNA启动子甲基化水平与早发急性冠脉综合征及其病变严重程度的关系
Relationship between ABCA1 DNA promoter methylation level and the risk and severity of premature acute coronary syndrome
摘要
Abstract
Objective To investigates the relationship between ATP binding cassette transporter A1(ABCA1)DNA methyla-tion level and the risk and severity of premature acute coronary syndrome(pACS).Methods A total of 90 patients with pACS admit-ted to the Cardiology Department of Characteristics Medical Center of Chinese People's Armed Police Force from May to December 2019 were selected as pACS group,and 88 healthy physical examinees from the health examination center were selected as control group.Pyrosequencing method was used to determine ABCA1 DNA methylation level in peripheral blood of the two groups,and the bi-ochemical indicators such as blood lipids and blood glucose,as well as the concentration of serum inflammatory markers were com-pared.Logistic regression was used to analyze the risk factors affecting the onset of pACS.Results The BMI,HbA1c,LDL,waist circumference,average ABCA1 DNA methylation level,and inflammatory markers including interleukin-1(IL-1β),C-reactive protein(CRP)and circulating free DNA/Neutrophil extracellular traps(cfDNA/NETs)in the pACS group were higher than those in the control group(all P<0.05),while the HDL-C level and ABCA1 pro-tein level were lower than those in the control group(all P<0.05).Further logistic regression analysis revealed that ABCA1 DNA methyl-ation level(OR=7.413,95%CI:2.070-26.554),HbA1c(OR=3.646,95%CI:1.008-13.182),LDL(OR=15.690,95%CI:1.123～69.233),cfDNA/NETs(OR=1.892,95%CI:1.374-2.604)and IL-1 β(OR=1.177,95%CI:1.011-1.370)were independent risk factors for pACS,while HDL(OR=0.107,95%CI:0.025-0.398)was a protective factor for pACS.In addition,studies showed a positive correlation between ABCA1 DNA methylation level and coronary Gensini scores in pACS patients(r=0.648,P<0.001).Conclusions The methylation level of ABCA1 DNA pro-moter is an independent risk factor for the occurrence of pACS and is related to the degree of coronary artery disease.Therefore,serum ABCA1-A promoter methylation level may be used as a biomarker to predict the risk of pACS.关键词
三磷酸腺苷结合盒转运蛋白A1/DNA甲基化/早发急性冠脉综合征/高密度脂蛋白胆固醇/Gensini评分Key words
ATP binding cassette transporter A1/DNA methylation/pACS/high-density lipoprotein cholesterol/Gensini score分类
医药卫生引用本文复制引用
安芳,李学文,杨丽荣,李覃,丛洪良,赵季红..ABCA1 DNA启动子甲基化水平与早发急性冠脉综合征及其病变严重程度的关系[J].武警医学,2024,35(6):461-466,6.基金项目
天津市救援医学临床研究中心子课题/天津市科技计划项目(15ZXLCSY00040) （15ZXLCSY00040）
