武警医学2024，Vol.35Issue(6)：474-479,6.
肠源性氧化三甲胺对缺血性心力衰竭大鼠预后的影响
Effect of intestinal-derived trimethylamine oxide levels on prognosis of ischemic heart fail-ure rats
摘要
Abstract
Objective To evaluate the effect of trimethylamine oxide(TMAO)on the prognosis of ischemic heart failure rats.Methods SD rats were randomly divided into normal control group,sham group,model group(HF)and intervention group(HF-DMB).Rats in HF and HF-DMB groups underwent myocardial infarction simulation via ligation,and cardiac function was assessed at 4 weeks(4 W),8 weeks(8 W)and 12 weeks(12 W)after surgery,respectively.The levels of Serum TMAO,BNP,CRP,LPS and IL-6 were detected,along with histological examination of myocardial tissue using HE and Masson staining.Fecal samples were al-so collected for 16S rDNA high-throughput sequencing analysis.Results Compared with Control group,HF group exhibited a signifi-cant decline in(LVEF)over time,the difference was most pronounced in the eighth week(27.68±6.41)%,also with a notable in-crease in circulating TMAO[(9534.02±202.75)nmol/L,(12713.06±243.66)nmol/L,(4970.95±168.15)nmol/L],BNP[(37.26±3.41)ng/L,(59.17±3.80)ng/L,(58.83±4.41)ng/L],CRP[(179.30±13.85)μg/L,(186.40±13.47)μg/L,(187.21±11.35)μg/L],LPS[(92.45±8.83)ng/L,(90.65±7.5)ng/L,(95.20±4.68)ng/L],IL-6[(97.57±3.48)pg/ml,(102.60±4.96)pg/ml,(107.70±4.70)pg/ml],levels compared with the control group(P<0.05).Myocardial pathological staining showed that more myocardial cells survived and the degree of fibrosis was reduced in HF-DMB group than in HF group.Sequencing analysis re-vealed increased microbial richness in the HF-DMB group compared with the HF group with increased Firmicutes and decreased Bacteroidetes at 8 weeks,resulting in a lowered Firmicutes/Bacteroidetes ratio.However,these changes were reversed in the HF-DMB-8W group.Conclusions Changes of circulating TMAO in ischemic heart failure rats are consistent with traditional heart failure evaluation markers.Specific in-hibition of circulating TMAO can improve various indexes of heart failure,indicating its potential clinical value in heart failure treatment and prognostic assessment.关键词
氧化三甲胺/心力衰竭/3,3-二甲基-1-丁醇/肠道菌群Key words
trimethylamine oxide/heart failure/3,3-dimethyl-1-butanol/gut microbiota分类
医药卫生引用本文复制引用
张芯,石蕊,倪健美,刘小伟,郭琼,王涛,王晓静,陈少伯..肠源性氧化三甲胺对缺血性心力衰竭大鼠预后的影响[J].武警医学,2024,35(6):474-479,6.基金项目
天津市应用基础研究项目(21JCQNJC01230) （21JCQNJC01230）
中心级创新团队课题(KYCXTD0502) （KYCXTD0502）
