医药导报2024，Vol.43Issue(7)：1050-1054,5.DOI:10.3870/j.issn.1004-0781.2024.07.004
两面针粗生物碱的富集
Enrichment of Crude Alkaloids from Zanthoxylum nitidum
摘要
Abstract
Objective To explore the enrichment method of the crude alkaloid of Zanthoxylum nitidum(Roxb.)DC.(Z.nitidum),which can inhibit the proliferative activity of human erythroid leukemia cells(HEL cells)in vitro,and to provide a scientific basis for the antitumor activity of Z.nitidum.Methods Z.nitidum was extracted using different concentrations of ethanol(60%,70%,80%,90%,and 100%)to determine the optimum extraction conditions according to the highest extraction rate.Static and dynamic adsorption determined the adsorption capacity of different macroporous resins(HPD 100,XDA-5,D101,HPD 722,HPD 400)on the ethanol extracts from Z.nitidum.The effects of crude alkaloids on the proliferation activity of HEL cells in each group were studied by the MTT method.Results Z.nitidum was extracted from 60%,70%,80%,90%,and 100%ethanol,and the extract with 60%ethanol concentration had the highest yield(11.937±0.002)%.Static and dynamic adsorption experiments showed that the adsorption capacity of the above five kinds of macroporous resins was 1∶500(extract of Z.nitidum∶macroporous resin).The crude alkaloids enriched by different macroporous resins have different effects on the proliferation activity of HEL cells.The inhibition rates(%)at a concentration of 80 μg·mL-1 of Z.nitidum were(59.62±3.46)%(XDA-5),(55.81±5.16)%(HPD 100),(59.33±1.33)%(D101),(79.63±1.02)%(HPD 722),and(83.97±0.38)%(HPD 400),respectively.HPD 400 macroporous resin-enriched crude alkaloids inhibited the proliferation of HEL cells most significantly(P<0.01).Conclusion The process of enriching the crude alkaloids of Z.nitidum using macroporous resin was simple and feasible,and the crude alkaloids enriched by HPD 400 showed significant inhibitory effects on HEL cells with good anti-leukemic activity.关键词
两面针/粗生物碱/提取与富集/大孔吸附树脂/抗白血病Key words
Zanthoxylum nitidum(Roxb.)DC/Crude alkaloids/Extraction and enrichment/Macroporous adsorption resin/Anti-leukemia分类
医药卫生引用本文复制引用
郑朴,徐兴莲,邓璐璐,李江,范艳华,穆淑珍..两面针粗生物碱的富集[J].医药导报,2024,43(7):1050-1054,5.基金项目
国家自然科学基金资助项目(31760097) （31760097）
贵州省科技计划项目(黔科合基础-ZK[2021]一般516) （黔科合基础-ZK[2021]一般516）
贵州省高校科技人才培养计划项目(黔教合KY字[2021]181). （黔教合KY字[2021]181）
