医药导报2024，Vol.43Issue(7)：1055-1060,6.DOI:10.3870/j.issn.1004-0781.2024.07.005
丹皮酚对大鼠肾间质纤维化的影响及其分子机制
Effect and Molecular Mechanism of Paeonol on Renal Interstitial Fibrosis in Rats
摘要
Abstract
Objective To study the effect and mechanism of paeonol(PAE)on renal interstitial fibrosis in rats.Methods The rats were randomly divided into sham operation(Sham)group,unilateral ureteral obstruction(UUO)group,PAE low dose(PAE-L)group,PAE medium dose(PAE-M)group,PAE high dose(PAE-H)group and irbesartan(IRB)group.Except for the Sham group,the UUO model was established in other groups.Each group was given a corresponding intervention for two weeks.Serum creatinine(Scr),blood urea nitrogen(BUN),serum 8-hydroxydeoxyguanosine(8-OHdG)levels,renal tissue superoxide dismutase(SOD),glutathione peroxidase(GPX)activities,α-smooth muscle actin(α-SMA),type Ⅰ collagen(Col-Ⅰ),fibronectin(FN),silent information regulator 1(SIRT1),nuclear factor E2-related factor 2(Nrf2)protein expression were detected;observe pathological changes of kidney tissue and calculate collagen volume fraction(CVF).Results Compared with the UUO group,the serum levels of Scr,BUN,and 8-OHdG in each dose group of PAE were decreased,the activities of SOD and GPX in kidney tissue were increased,the positive expressions of α-SMA,Col-Ⅰ and FN in kidney tissue were decreased,and the protein expressions of SIRT1 and Nrf2 were increased.Masson staining showed a decrease of CVF in renal tissue(all P<0.05),and HE staining showed a different degree of improvement in pathological changes such as inflammatory cell infiltration and tubular dilatation in renal tissue;PAE improves renal interstitial fibrosis in rats in a dose-dependent manner(P<0.05),and the effect of large dose PAE on renal interstitial fibrosis in rats was similar to that of IRB.Conclusion PAE can alleviate UUO-induced rat renal interstitial fibrosis and oxidative stress,and improve rat renal function.And this mechanism may be related to the activation of the SIRT1/Nrf2 signaling pathway.关键词
丹皮酚/肾间质纤维化/单侧输尿管梗阻/SIRT1/Nrf2信号通路Key words
Paeonol/Renal interstitial fibrosis/Unilateral ureteral obstruction/SIRT1/Nrf2 signaling pathway分类
医药卫生引用本文复制引用
高海洋,刘健,张立国,张金存,陈曦,关晓海,曹凤宏,康绍叁,高伟兴,王磊,么安亮..丹皮酚对大鼠肾间质纤维化的影响及其分子机制[J].医药导报,2024,43(7):1055-1060,6.基金项目
河北省医学科学研究课题计划项目(20210159). （20210159）
