医药导报2024，Vol.43Issue(7)：1112-1119,8.DOI:10.3870/j.issn.1004-0781.2024.07.016
基于化学成分与药理作用评价当归-黄芪药对配方颗粒汤剂与传统汤剂的差异
Evaluation of the Difference Between Angelica-Astragalus Medicine Pair Dispensing Granules Decoction and Traditional Decoction Based on Chemical Composition and Pharmacological Action
摘要
Abstract
Objective To evaluate the differences in chemical composition and pharmacological effects between Angelica-Astragalus medicine pair decoction(DGD)and traditional decotion,and to provide a reference for the rational clinical application of Danggui Buxue decoction.Methods With the two comparison methods of unified and non-uniform raw material source batches,we set up different sample groups,established characteristic maps by HPLC,and evaluated the chemical components based on the similarity of characteristic maps,component types,index component content,common peak area,and other factors.The efficacy of the drug was evaluated in the hemorrhagic blood deficiency model mice.Results ①The similarity of the feature map between the DGD and TD was high(similarity was greater than 0.87).②The number of chromatographic peaks was inconsistent.Traditional decoction from self-purchased decoction pieces,or traditional decoction-Factory A decoction pieces had a total of 12 chromatographic peaks each.The DGD of Factory A had a total of 15 chromatographic peaks.There were 10 chromatographic peaks in the DGD of Factory B.③The contents of ferulic acid and calycosin 7-O-glucoside(CG)in DGD of Factory A were higher than those in traditional decoction(P<0.05,n=3).There was no significant difference between DGD and TD ferulic acid content in Factory B,but the content of CG was lower than that in traditional decoction(P<0.05).④The total area of common peaks in DGD was different from that in TD.The relative total ratios of the contents of the components in the self-purchased traditional decoction pieces,the traditional decoction pieces of Factory A,the formula granules of Factory A,and the formula granules of Factory B were 1.00,0.96,2.14,0.60,respectively.⑤Both DGD and traditional decoction could significantly promote the recovery of hemoglobin and red blood cells in hemorrhagic anemia model mice(P<0.01);Compared with the model control group,there was a significantly difference(P<0.05)except for the DGD group of Plant B.There was no significant difference between DGD and TD of Plant A,but there was a very significant difference between DGD and TD of Plant B(P<0.01).Conclusion Whether the raw material source batch is consistent or not,DGD and TD have certain differences in chemical composition.In terms of pharmacological effect,DGD,prepared from a unified batch of decoction pieces,has similar efficacy to traditional decoction in alleviating hemorrhagic anemia.There are certain differences in the pharmacological effects between DGD prepared from different batches of decoction pieces and traditional decoctions.The differences caused by the different preparation processes of the same source batch of prepared slices were compared,and the quality differences of the formula granules from different manufacturers were caused by the different source batches of prepared slices and different preparation processes,indicating the necessity and urgency of the country to formulate a unified quality standard for formula granules and related process specifications.关键词
当归-黄芪药对/当归补血汤/传统汤剂/配方颗粒Key words
Angelica-astragalus medicine pair/Danggui Buxue decoction/Traditional decotion/Dispensing granules分类
医药卫生引用本文复制引用
姚静,刘瑞新,麻利杰,任延娜,李啸鹏,施钧瀚,桂新景,张璐,王青晓,李学林..基于化学成分与药理作用评价当归-黄芪药对配方颗粒汤剂与传统汤剂的差异[J].医药导报,2024,43(7):1112-1119,8.基金项目
国家自然科学基金资助项目(81773892) （81773892）
河南省中医管理局国家中医临床研究基地科研专项(2021JDZY036) （2021JDZY036）
河南省中医管理局国家中医临床研究基地科研专项(2018JDZX039) （2018JDZX039）
河南省科技攻关计划项目(172102310135). （172102310135）
