中国病理生理杂志2024，Vol.40Issue(6)：989-996,8.DOI:10.3969/j.issn.1000-4718.2024.06.004
心痛泰颗粒对动脉粥样硬化ApoE-/-小鼠ox-LDL、ICAM-1和VCAM-1表达的影响
Effects of Xin-Tong-Tai granule on expression of ox-LDL,ICAM-1 and VCAM-1 in ApoE-/-mice with atherosclerosis
摘要
Abstract
AIM:To investigate the effects and mechanism of Xin-Tong-Tai granule on oxidized low-density li-poprotein(ox-LDL),intercellular adhesion molecule-1(ICAM-1)and vascular cell adhesion molecule-1(VCAM-1)in ApoE-/-mice with atherosclerosis(AS).METHODS:A total of 72 SPF-grade healthy male ApoE-/-mice aged 6～8 weeks were fed with high-fat diet for 12 weeks to replicate AS models,and 12 SPF-grade healthy male C57BL/6J wild mice were fed with ordinary diet as the control group.After the corresponding drugs were administered for 8 weeks,the body weight and general condition of mice in each group were observed.The serum levels of total cholesterol(TC),triglyceride(TG),high-density lipoprotein cholesterol(HDL-C)and low-density lipoprotein cholesterol(LDL-C)were detected by biochemi-cal kits.The pathological structures of aorta were observed by HE and oil red O staining.The levels of serum ox-LDL and aortic ICAM-1 and VCAM-1 were detected by ELISA.The protein levels of NADPH oxidase 4(NOX4),NOX subunit p22phox,inhibitor of κB kinase-α(IKK-α),IKK-β and nuclear factor-κB(NF-κB)in aorta were detected by Western blot.RESULTS:Compared with control group,the mice in model group showed increased body weight(P<0.05),dull and lo-cal shedding hair,slow grasping response,increased serum TC,TG and LDL-C levels,decreased serum HDL-C level(P<0.05),increased the levels of serum ox-LDL and aortic ICAM-1 and VCAM-1(P<0.05),and increased protein expres-sions of NOX4,p22phox,IKK-α,IKK-β and NF-κB in aorta(P<0.05).Compared with model group,the body weight of mice in each treatment group decreased(P<0.05),the hair loss and the response flexibility were also improved.The se-rum levels of TC,TG and LDL-C decreased and HDL-C increased(P<0.05).The levels of serum ox-LDL and aortic ICAM-1(except the low-dose Xin-Tong-Tai granule group)and VCAM-1 decreased(P<0.05).The protein levels of NOX4,p22phox,IKK-α,IKK-β and NF-κB in aorta decreased(P<0.05).HE and oil red O staining showed that typical AS plaques could be seen in blood vessels of the model group,and the red-stained areas were widely distributed.The above lesions were alleviated to different degrees in each treatment group compared with model group.CONCLUSION:Xin-Tong-Tai granule reduces the atherosclerotic plaque area of ApoE-/-mice induced by high-fat diet,decreased serum TC,TG and LDL-C levels,increased HDL-C level,decreased the levels of serum ox-LDL and aorta ICAM-1 and VCAM-1,and inhibited protein expression of NOX4,p22phox,IKK-α and IKK-β in the aorta,thereby attenuating AS.关键词
动脉粥样硬化/心痛泰颗粒/氧化型低密度脂蛋白/细胞间黏附分子1/血管细胞黏附分子1Key words
atherosclerosis/Xin-Tong-Tai granule/oxidized low-density lipoprotein/intercellular adhesion molecule-1/vascular cell adhesion molecule-1分类
医药卫生引用本文复制引用
曾清华,尹紫薇,黄爱思,陈景怡,郭志华,魏佳明..心痛泰颗粒对动脉粥样硬化ApoE-/-小鼠ox-LDL、ICAM-1和VCAM-1表达的影响[J].中国病理生理杂志,2024,40(6):989-996,8.基金项目
湖南省自然科学基金项目(No.2021JJ30492) （No.2021JJ30492）
湖南中医药大学中医学一流学科重点项目(No.2021ZYX14) （No.2021ZYX14）
湖南省研究生科研创新项目(No.2022CX133 （）
No.2023CX178) （）
湖南中医药大学"一方"研究生创新项目(No.2022YF05) （No.2022YF05）
湖南省中医药科研项目重点课题(No.2021029) （No.2021029）
湖南省教育厅科研项目重点课题(No.20A384) （No.20A384）
