中国病理生理杂志2024，Vol.40Issue(6)：1052-1061,10.DOI:10.3969/j.issn.1000-4718.2024.06.011
PCGF3在口腔鳞状细胞癌中的表达及其对细胞活力和凋亡的影响
Expression of PCGF3 in oral squamous cell carcinoma and its effect on cell viability and apoptosis
摘要
Abstract
AIM:To investigate the effect of polycomb group ring finger 3(PCGF3)on the viability and apop-tosis of OSCC cells and its potential mechanism.METHODS:The data were downloaded from the online public database to predict the expression of PCGF3 in OSCC and its relationship with pathological stage survival rate.Fifty pairs of OSCC tissues and adjacent tissues were selected to analyze the relationship between the expression of PCGF3 and the survival rate of patients.The expression level of PCGF3 in the specimens was detected by IHC,and the extracted cancer and adja-cent tissues were homogenized to analyze its mRNA expression.The mRNA and protein expression levels of PCGF3 in the cells were analyzed by RT-qPCR and Western blot experiments,and the protein expressions of PCGF3,p-PI3K,p-AKT,cyclin D1 and cleaved caspase-3(cl-caspase-3)were further analyzed.In addition,the effects of PCGF3 on cell viability and apoptosis were also detected.Finally,subcutaneous tumor models in nude mice were constructed to verify the above experiments.RESULTS:PCGF3 was highly expressed in OSCC and was closely related to pathological stage,pathologi-cal grade,and survival rate of patients.PCGF3 was highly expressed in OSCC tissues and cell lines in the network online databases.Western blot results showed that the expressions of PCGF3,p-PI3K,p-AKT,cyclin D1 and cl-caspase-3 were decreased and increased respectively after knocking down or overexpressing PCGF3 in OSCC cells.And cell viability and apoptosis were affected.The volume and weight of tumors in sh-PCGF3 group were lower than those in sh-NC group,and HE results showed that the nuclear atypotype of tumor cells in sh-PCGF3 group was less than that in sh-NC group.CON-CLUSION:PCGF3 can significantly affect the viability and apoptosis of OSCC cells,and its potential mechanism is to ac-tivate the PI3K/AKT signaling pathway to participate in the proliferation and apoptosis of OSCC cells.关键词
口腔鳞状细胞癌/多梳环指蛋白3/PI3K/AKT信号通路Key words
oral squamous cell carcinoma/polycomb group ring finger 3/PI3K/AKT signaling pathway分类
医药卫生引用本文复制引用
王艳霞,李欢,张雪莹,胥欣,杨杰..PCGF3在口腔鳞状细胞癌中的表达及其对细胞活力和凋亡的影响[J].中国病理生理杂志,2024,40(6):1052-1061,10.基金项目
山东省中医药科技发展计划项目(No.M-2022235) （No.M-2022235）
山东省优质专业学位教学案例库项目(No.SDYAL2022149) （No.SDYAL2022149）
