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贝母素乙通过下调CDK2/CDK4/CDK6和cyclin D1表达抑制人结肠腺癌SW480细胞活力

杨霞朱羿龙金宁一李雅茹李玥毛泓月白冰李一权韩继成万怡宁谢诗敏

中国病理生理杂志2024，Vol.40Issue(6)：1070-1077,8.

中国病理生理杂志2024，Vol.40Issue(6)：1070-1077,8.DOI:10.3969/j.issn.1000-4718.2024.06.013

贝母素乙通过下调CDK2/CDK4/CDK6和cyclin D1表达抑制人结肠腺癌SW480细胞活力

Peiminine inhibits viability of human colonic adenocarcinoma SW480 cells by down-regulating expression of CDK2/CDK4/CDK6 and cyclin D1

杨霞 ¹朱羿龙 ²金宁一 ³李雅茹 ¹李玥 ⁴毛泓月 ⁵白冰 ⁴李一权 ⁴韩继成 ⁴万怡宁 ⁴谢诗敏⁴

作者信息

1. 延边大学医学院病理学与病理生理学教研室,吉林延吉 136200
2. 长春中医药大学院士工作室,吉林长春 130117||中国农业科学研究院长春兽医研究所,吉林长春 130122
3. 延边大学医学院病理学与病理生理学教研室,吉林延吉 136200||中国农业科学研究院长春兽医研究所,吉林长春 130122
4. 长春中医药大学院士工作室,吉林长春 130117
5. 中国农业科学研究院长春兽医研究所,吉林长春 130122
折叠

摘要

Abstract

AIM:This study examined the inhibitory effect of peiminine on the human colonic adenocarcino-ma cell line SW480 and explored the underlying mechanisms.METHODS:SW480 and human normal colonic epithelial CCD-841CoN cells were treated with different concentrations of peiminine and subjected to the CCK-8 assay to select the optimal treatment time and concentration of the compound.SW480 cell migration and invasion were evaluated by the wound-healing and Transwell assays.Cell cycle progression was analyzed by flow cytometry.The expression levels of cell cycle-related proteins were examined by Western blot.SW480 xenograft tumor model was established in nude mice to ex-amine the effect of peiminine on tumor growth and the expression of cell cycle-related proteins in vivo.RESULTS:Peimi-nine(110 mg/L)significantly inhibited the proliferation of SW480 cells compared with the control group(P<0.01),caused cell cycle arrest at G1 phase,and significantly downregulated the expression of cyclin dependent kinase 2(CDK2),CDK4,CDK6,cyclin D1,p-Rb/Rb,E2F1,E2F3,and E2F4(P<0.05).Peiminine inhibited SW480 xenograft tumor growth,prolonged the survival of model mice,and affected the expression of CDK2,CDK4,CDK6,and cyclin D1 in tu-mor tissues.CONCLUSION:Peiminine promotes G1 phase arrest by down-regulating the expression of CDK2,CDK4,CDK6,and cyclin D1,thereby inhibiting the proliferation of SW480 cells.

关键词

SW480细胞/贝母素乙/周期蛋白依赖性激酶/细胞周期

Key words

SW480 cells/peiminine/cyclin-dependent kinases/cell cycle

分类

医药卫生

引用本文复制引用

杨霞,朱羿龙,金宁一,李雅茹,李玥,毛泓月,白冰,李一权,韩继成,万怡宁,谢诗敏..贝母素乙通过下调CDK2/CDK4/CDK6和cyclin D1表达抑制人结肠腺癌SW480细胞活力[J].中国病理生理杂志,2024,40(6):1070-1077,8.

基金项目

吉林省自然科学基金资助项目(No.YDZJ202401687ZYT) （No.YDZJ202401687ZYT）

吉林省中医药科技项目(No.2024255) （No.2024255）

吉林省科技发展计划项目(No.20220508075RC) （No.20220508075RC）

中国病理生理杂志

OA北大核心CSTPCD

ISSN：1000-4718

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