中国病理生理杂志2024，Vol.40Issue(6)：1078-1084,7.DOI:10.3969/j.issn.1000-4718.2024.06.014
干扰Gal-1通过TGF-β通路抑制人乳腺癌MDA-MB-231细胞的EMT和迁移
Interference with Gal-1 inhibits EMT and migration of human breast cancer MDA-MB-231 cells via TGF-β pathway
摘要
Abstract
AIM:To explore the effect and mechanism of the interfering Gal-1 on epithelial-mesenchymal transition(EMT),migration and proliferation in MDA-MB-231 cells via transforming growth factor-β(TGF-β)pathway.METHODS:The stable cell lines(shGal-1)which Gal-1 expression were inhibited completely and their control cell lines were used as experimental cells.Western blot assay was used to detect the effects of shGal-1 on EMT process of MDA-MB-231 cells after TGF-β treatment;The effect of shGal-1 on cell migration and invasion after TGF-β treatment was verified by cell scratch and transwell test;The effect of shGal-1 on the TGF-β pathway related proteins were detected by western blot;Finally,the effect of shGal-1 on cell proliferation was detected by MTT and western blot.RESULTS:shGal-1 inhib-ited TGF-β-mediated EMT in MDA-MB-231 cells and regulated phosphorylation of pathway signaling molecules(ERK,AKT and GSK3β);shGal-1 could inhibit the proliferation of MDA-MB-231 cells.CONCLUSION:shGal-1 can inhibit the TGF-β-mediated EMT,migration and proliferation of MDA-MB-231 cells.关键词
半乳糖凝集素1/上皮-间充质转化/转化生长因子β/MDA-MB-231细胞/细胞迁移/细胞增殖Key words
Galectin-1/epithelial-mesenchymal transition/transforming growth factor-β/MDA-MB-231 cells/cell migration/cell proliferation分类
医药卫生引用本文复制引用
任世忠,秦旭勇,周国利,赵薇,曹淑俊,苗振宇,李成萍..干扰Gal-1通过TGF-β通路抑制人乳腺癌MDA-MB-231细胞的EMT和迁移[J].中国病理生理杂志,2024,40(6):1078-1084,7.基金项目
山东省自然科学基金资助项目(No.ZR2020QC064) （No.ZR2020QC064）
聊城大学科技处博士科研启动资金(No.318051604) （No.318051604）
