一株野鸭源禽副黏病毒6型的基因组特征分析OA

In order to identify the biological characteristics of avian paramyxovirus type 6(APMV-6)present in wild waterfowl in China,one APMV-6 strain(wild duck/Shandong/W683/2020)isolated in 2020 was sequenced and analyzed.The results showed that the genome of the isolate was a single negative-strand RNA with the genome length of 16 230 nt and the structure of 3'-N-P-M-F-SH-HN-L-5',the length of 3'leader sequence and 5'trailing sequence was 55 and 54 nt,respectively,and the length of each gene interval sequence varied from 2 to 63 nt;the R value between it with vaccine strain La Sota was 0.167,and the antigenicity was greatly different;the cleavage site of F protein was 104IREPR↓L109,showing the typical low pathogenic APMV molecular characteristic.Homology analysis showed that it was the highest identical(96.0%)to Taiwan strain(MZ802803),but 63.1%～70.2%to other serotypes of APMV,locating in the same branch as the APMV-6 strain isolated from antheriformes in China(MZ802803).A basis was laid for further understanding of the genome and biological characteristics of APMV-6 derived from wild birds in China.