中国当代儿科杂志2024，Vol.26Issue(6)：575-583,9.DOI:10.7499/j.issn.1008-8830.2309129
4～6岁阻塞性睡眠呼吸暂停低通气综合征患儿肠道代谢产物特征及临床价值分析
Characteristics and clinical value of intestinal metabolites in children aged 4-6 years with obstructive sleep apnea-hypopnea syndrome
摘要
Abstract
Objective To study the characteristics and clinical value of intestinal metabolites in children aged 4-6 years with obstructive sleep apnea-hypopnea syndrome(OSAHS).Methods A total of 31 children aged 4-6 years with OSAHS were prospectively enrolled as the test group,and 24 healthy children aged 4-6 years were included as the control group.Relevant clinical indicators were recorded.Fecal samples were collected,and non-targeted metabolomics analysis using liquid chromatography-mass spectrometry was performed to detect all metabolites.Results A total of 206 metabolites were detected,mainly amino acids and their derivatives.There was a significant difference in the overall composition of intestinal metabolites between the test and control groups(P<0.05).Eighteen different metabolites were selected,among which six(N-acetylmethionine,L-methionine,L-lysine,DL-phenylalanine,L-tyrosine,and L-isoleucine)had receiver operating characteristic curve areas greater than 0.7 for diagnosing OSAHS.Among them,N-acetylmethionine had the largest area under the curve,which was 0.807,with a sensitivity of 70.83%and a specificity of 80.65%.Correlation analysis between different metabolites and clinical indicators showed that there were positive correlations between the degree of tonsil enlargement and enterolactone,between uric acid and phenylacetaldehyde,between blood glucose and acetylmethionine,and between cholesterol and 9-bromodiphenyl and procaine(P<0.05).There were negative correlations between the degree of tonsil enlargement and N-methyltyramine,aspartate aminotransferase and indolepropionic acid and L-isoleucine,between alanine aminotransferase and DL-phenylalanine,between indolepropionic acid and L-isoleucine,between uric acid and hydroxyquinoline,and between urea nitrogen and N,N-dicyclohexylurea(P<0.05).The metabolic functional pathways affected by differential metabolites mainly included riboflavin metabolism,arginine and proline metabolism,pantothenic acid and coenzyme A biosynthesis,cysteine and methionine metabolism,lysine degradation and glutathione metabolism.Conclusions Intestinal metabolites and metabolic functions are altered in children aged 4-6 years with OSAHS,primarily involving amino acid metabolism disorders.The screened differential intestinal metabolites have potential screening and diagnostic value as biomarkers for OSAHS.关键词
阻塞性睡眠呼吸暂停低通气综合征/肠道代谢产物/生物标志物/代谢功能/儿童Key words
Obstructive sleep apnea-hypopnea syndrome/Intestinal metabolite/Biomarker/Metabolic function/Child引用本文复制引用
陈悦,卢燕波,吴军华,邱海燕..4～6岁阻塞性睡眠呼吸暂停低通气综合征患儿肠道代谢产物特征及临床价值分析[J].中国当代儿科杂志,2024,26(6):575-583,9.基金项目
浙江省医药卫生科技计划(2024KY1576) （2024KY1576）
第一批宁波市临床医学研究中心依托单位(2019A21002) （2019A21002）
宁波市社会公益研究项目(2021S100) （2021S100）
宁波市医学重点学科建设计划(2022-B17) （2022-B17）
宁波市医疗卫生高端团队项目(2022020405). （2022020405）
