中国动物检疫2024，Vol.41Issue(6)：107-114,8.DOI:10.3969/j.issn.1005-944X.2024.06.019
5种猪场常用消毒剂对PCV2和PRV的消毒效果评价
Evaluation on the Disinfection Effects of 5 Disinfectants Commonly Used by Swine Farms against PCV2 and PRV
摘要
Abstract
In order to evaluate the safety of 5 disinfectants containing iodine,quaternary ammonium salts,potassium bisulfate,chlorine dioxide or glutaraldehyde commonly used by swine farms and their disinfection effects on viruses,their working concentrations were designed as high,medium and low under laboratory and simulated farm conditions according to the dilution ratios recommended by their instructions.After the disinfectants reacting with PCV2 and PRV for different time,the reaction product was inoculated into cells,and cell lesions were observed,the viral inactivation rate was calculated by TCID50,and the viral nucleic acid content was detected by fluorescence quantitative PCR.The results showed that,the disinfectants containing iodine and chlorine dioxide were less toxic to the cells,but those containing glutaraldehyde was on the contrary.All the 5 disinfectants could kill PCV2 and PRV to certain extent,the lower the concentration was,the longer the inactivation period became;under laboratory conditions,the disinfectants containing iodine,potassium bisulfate or chlorine dioxide,with stronger disinfection,could inactivate 100%viruses within 10 minutes at high and medium concentrations,while under simulated farm conditions,the disinfectants containing iodine were poor in disinfection,and those containing quaternary ammonium salts were poor in the removal of nucleic acids.In conclusion,all the 5 disinfectants could be used for disinfection on swine farms according to actual conditions and requirements;higher concentrations and enough disinfection time should be ensured as much as possible under the premise of ensuring safety.In the study,the correlation of inactivation effect of 5 disinfectants and the working concentrations and disinfection time were determined,providing a reference for the selection and use of disinfectants on swine farms.关键词
消毒剂/猪伪狂犬病病毒/猪圆环病毒/消毒效果Key words
disinfectant/PRV/PCV/disinfection effect分类
农业科技引用本文复制引用
董林,谢金文,刘吉山,曲光刚,王艳萍,魏凤,沈志强..5种猪场常用消毒剂对PCV2和PRV的消毒效果评价[J].中国动物检疫,2024,41(6):107-114,8.基金项目
山东省自然科学基金项目(ZR2023MC212) （ZR2023MC212）
滨州市农社领域科技创新政策引导计划项目(2022KTPY027) （2022KTPY027）
中央引导地方科技发展专项资金项目(YDZX2022008) （YDZX2022008）
