中国农业科学2024，Vol.57Issue(12)：2454-2466,13.DOI:10.3864/j.issn.0578-1752.2024.12.014
2021-2022年上海地区大米、玉米、大豆和面粉中多种真菌毒素混合污染特征和交互作用分析
Study on Characterization and Interaction Analysis of Co-Contamination of Multi-Mycotoxins in the Flours of Rice,Maize,Soybean and Wheat Flour in Shanghai from 2021 to 2022
摘要
Abstract
[Objective]The co-contamination of multiple mycotoxins in grain food ingredients has become one of most concerned problem of food safety.In this study,the characteristics and interactions of co-contamination of 14 mycotoxins in the flours of rice,maize,soybean and wheat in Shanghai were explored,in order to provide the basic reference for safety assessment,early warning and supervision of mycotoxins,and development of efficient monitoring measures.[Method]The content of 14 mycotoxins in 621 substrates of rice,maize,soybean and wheat flour from Shanghai were determined by ultra performance liquid chromatography tandem-triple quadrupole mass spectrometry(UPLC-MS/MS).The co-contaminated characteristics and interaction probabilities of multi-mycotoxin was analyzed by the common contamination index(CCEI),correlation cluster analysis(CCA),one-way ANOVA(one-way ANOVA),and the JAVA procedure.[Result]The main exposure of mycotoxins in the grain ingredients in Shanghai regions included ochratoxin(OTA),deoxynivalenol(DON),aflatoxins(AFs)and fumonisins(FUMs).Among them,rice was mainly contaminated by OTA and DON,maize was mainly contaminated by FUMs and DON,soybean was mainly contaminated by DON and AFs,and wheat flour was mainly contaminated with DON and its derivatives.Based on the safety limit standards of mycotoxins,the total excess rate was lower than 2.09％in four kinds of grain food ingredients,but the co-contamination rate of two or more kinds of mycotoxins were upper than 87.76％.Moreover,there was much higher interaction rates(25.44％)among different mycotoxins,such as FB1+FB2,DON+FB1,OTA+FB1,OTA+DON,AFM2+OTA,and DON+FB1+FB2.Among the higher interaction rates groups,the most of the mycotoxins cooccurrence showed weaker correlations,but the co-combinations of FB1+FB3,AFB1+DON,DON+15-ADON were all showed the significant moderate correlation.[Conclusion]The overall contaminated level and excessive rates of mycotoxins in the flours of rice,maize,soybean,and wheat flour from Shanghai was relatively low.However,the proportion of single samples contaminated by multi-mycotoxins was higher,and along with the difference of sample types.As a complex problem of the mycotoxin contamination in the flours of rice,maize,soybean and wheat,this study provided an important reference for the prevention and control of the co-occurrence of multi-mycotoxins in commercially available grain food ingredients in the future.关键词
真菌毒素/大米/玉米/黄豆/面粉/混合污染/交互性分析/上海地区Key words
mycotoxin/rice/maize/soybean/wheat flour/co-combination/interaction analysis/Shanghai region引用本文复制引用
朱佐银,赵含珂,程海生,韩梦怡,裘知,王杰,周新丽,杨俊花..2021-2022年上海地区大米、玉米、大豆和面粉中多种真菌毒素混合污染特征和交互作用分析[J].中国农业科学,2024,57(12):2454-2466,13.基金项目
上海市科委社会发展科技攻关项目(21DZ1201300) （21DZ1201300）
